Intracellular Polysaccharides of Aspergillus cristatus from Fuzhuan Brick Tea Leverage the Gut Microbiota and Repair the Intestinal Barrier to Ameliorate DSS-Induced Colitis in Mice,Journal of Agricultural and Food Chemistry

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Intracellular Polysaccharides of Aspergillus cristatus from Fuzhuan Brick Tea Leverage the Gut Microbiota and Repair the Intestinal Barrier to Ameliorate DSS-Induced Colitis in Mice
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-05-18 , DOI: 10.1021/acs.jafc.3c00611
Zhiyong Xie ₁ , Ziqi Zeng ₁ , Guijie Chen ₁ , Wei Dong ₁ , Yujia Peng ₁ , Weiqi Xu ₁ , Yi Sun ₁ , Xiaoxiong Zeng ₁ , Zhonghua Liu _{2,

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Affiliation

The intracellular polysaccharides of Aspergillus cristatus (IPSs) from Fuzhuan brick tea have been demonstrated to improve immune function linked to modulating the gut microbiota. Herein, to further investigate the efficacy of IPSs to maintain gut homeostasis, the protection of the purified fraction of IPSs (IPSs-2) on the mice with colitis induced by dextran sulfate sodium (DSS) and the underlying mechanisms were explored in this study. The results revealed that IPSs-2 alleviated the typical symptoms of colitis and suppressed the excessive inflammatory mediators, regulating the genes related to inflammatory responses in the colon at the mRNA level. Meanwhile, IPSs-2 treatment reinforced the intestinal barrier function by ameliorating the DSS-induced histological injury, facilitating the differentiation of goblet cells to enhance Mucin-2 generation, and enhancing the expression of tight junction proteins to alleviate colitis. In addition, IPSs protected against colitis by promoting the production of short-chain fatty acids (SCFAs), the activation of SCFAs receptors, and the leverage of the gut microbiota via the enrichment of Bacteroides, Parabacteroides, Faecalibacterium, Flavonifractor_plautii, and Butyricicoccus, linking with reducing inflammation and repairing intestinal barrier function. Overall, our research revealed the therapeutic potential of IPSs-2 as a prebiotic for attenuating inflammatory bowel disease and provided a rationale for future investigation.

中文翻译：

茯砖茶中的冠曲霉细胞内多糖利用肠道微生物群和修复肠道屏障改善 DSS 诱导的小鼠结肠炎

冠曲霉胞内多糖来自茯砖茶的 (IPS) 已被证明可以改善与调节肠道微生物群相关的免疫功能。在此，为了进一步研究 IPSs 维持肠道稳态的功效，本研究探讨了 IPSs 纯化部分 (IPSs-2) 对葡聚糖硫酸钠 (DSS) 诱导的结肠炎小鼠的保护作用及其潜在机制。结果表明，IPSs-2可减轻结肠炎的典型症状，抑制过多的炎症介质，在mRNA水平上调节结肠炎症反应相关基因。同时，IPSs-2 治疗通过改善 DSS 诱导的组织学损伤来增强肠屏障功能，促进杯状细胞的分化以增强 Mucin-2 的产生，并增强紧密连接蛋白的表达以减轻结肠炎。此外，IPSs 通过促进短链脂肪酸 (SCFAs) 的产生、SCFAs 受体的激活和肠道微生物群的杠杆作用来预防结肠炎通过丰富拟杆菌属、副杆菌属、粪杆菌属、Flavonifractor_plautii和丁酸球菌属，与减少炎症和修复肠道屏障功能有关。总体而言，我们的研究揭示了 IPSs-2 作为减轻炎症性肠病的益生元的治疗潜力，并为未来的研究提供了理论依据。

更新日期：2023-05-18

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