It has been reported that epigallocatechin-3-O-(3″-O-methyl)-gallate (EGCG3″Me) and the EGCG3″Me-rich green tea (Camellia sinensis L.) cultivar ‘Benifuuki’ exhibit antiallergic effects. The objective of this study was to investigate the effect of various tea leaf catechins on histamine release from murine bone marrow mast cells (BMMC). At a dose of 50 μg/mL, the rank order of histamine release inhibition was observed to be epicatechin-3-O-(3″-O-methyl)-gallate (ECG3″Me) > gallocatechin-3-O-(3″-O-methyl)-gallate (GCG3″Me) > EGCG3″Me > gallocatechin-gallate (GCG) > catechin-gallate (CG) > EGCG > epicatechin-gallate (ECG) > epigallocatechin (EGC) > gallocatechin (GC). Of the various tea cultivars analyzed by HPLC, the greatest content of ECG3″Me was found in the third crop of ‘Benifuuki’ (1.05% dry weight). Moreover, ECG3″Me content was positively correlated with EGCG3″Me content in ‘Benifuuki’ tea leaves. In an assay of mixtures of ECG3″Me and EGCG3″Me, inhibitory activity (50 μg/mL in total) was increased as the content of ECG3″Me increased. This suggests that ECG3″Me might link to the antiallergic effect of ‘Benifuuki’ tea, as has been reported for EGCG3″Me.

中文翻译：

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表儿茶素-3- Ö - （3“ - Ø甲基）-gallate内容在不同茶树品种（茶树L.）及体外抑制作用组胺释放

据报道，表没食子儿茶素-3 - O-（3″ -O-甲基）-没食子酸酯（EGCG3″ Me）和富含EGCG3″ Me的绿茶（山茶）表现出抗过敏​​作用。这项研究的目的是研究各种茶叶儿茶素对小鼠骨髓肥大细胞（BMMC）中组胺释放的影响。在50μg/ mL的剂量下，观察到组胺释放抑制的等级顺序为表儿茶素-3 -O-（3″ -O-甲基）-没食子酸酯（ECG3″ Me）> gallocatechin-3- O-（3 '' -O-甲基）-没食子酸酯（GCG3″ Me）> EGCG3″ Me>没食子儿茶素没食子酸酯（GCG）>儿茶素没食子酸酯（CG）> EGCG>表儿茶素没食子酸酯（ECG）>没食子儿茶素（EGC）>没食子儿茶素（GC）。通过HPLC分析的各种茶品种中，在“ Benifuuki”的第三茬（干重1.05％）中发现了最大含量的ECG3″ Me。此外，'Benifuuki'茶叶中的ECG3″ Me含量与EGCG3″ Me含量呈正相关。在ECG3″ Me和EGCG3″ Me混合物的测定中，抑制活性（总计50μg/ mL）随着ECG3″ Me含量的增加而增加。这表明ECG3″ Me可能与'Benifuuki'茶的抗过敏作用有关，正如有关EGCG3″ Me的报道。