Biological biogas upgrading has been well-proven to be a promising approach for renewable bioenergy recovery, but hydrogen (H₂)-assisted ex-situ biogas upgrading is hindered by a large solubility discrepancy between H₂ and carbon dioxide (CO₂). This study established a new dual-membrane aerated biofilm reactor (dMBfR) to improve the upgrading efficiency. Results showed that dMBfR operated at 1.25 atm H₂ partial pressure, 1.5 atm biogas partial pressure, and 1.0 d hydraulic retention time could significantly improve the efficiency. The maximum methane purity of 97.6%, acetate production rate of 34.5 mmol L⁻¹d⁻¹, and H₂ and CO₂ utilization ratios of 96.5% and 96.3% were achieved. Further analysis showed that the improved performances of biogas upgrading and acetate recovery were positively correlated with the total abundances of functional microorganisms. Taken together, these results suggest that the dMBfR, which facilitates the precise CO₂ and H₂ supply, is an ideal approach for efficient biological biogas upgrading.

生物沼气升级已被充分证明是可再生生物能源回收的一种有前途的方法，但氢气 (H ₂ ) 辅助的异地沼气升级受到 H ₂和二氧化碳 (CO ₂ ) 之间较大溶解度差异的阻碍。本研究建立了一种新型双膜曝气生物膜反应器（dMBfR）以提高升级效率。结果表明，dMBfR 在 1.25 atm H ₂分压、1.5 atm 沼气分压和 1.0 d 水力停留时间下运行可显着提高效率。最高甲烷纯度97.6%，乙酸产率为34.5 mmol L ⁻¹ d ⁻¹，H ₂和CO₂利用率分别达到96.5%和96.3%。进一步分析表明，沼气提质和醋酸盐回收性能的提高与功能微生物的总丰度呈正相关。_{综上所述，这些结果表明，有助于精确供应 CO 2}和 H ₂的 dMBfR是高效生物沼气升级的理想方法。