Screening of Eight Biofortified Rice Varieties Based on Total Content of Zinc and Iron as Well as Its Uptake by Caco-2 Cells: An In Vitro Cellular Model,ACS Physical Chemistry Au

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Screening of Eight Biofortified Rice Varieties Based on Total Content of Zinc and Iron as Well as Its Uptake by Caco-2 Cells: An In Vitro Cellular Model
ACS Physical Chemistry Au ( IF 3.7 ) Pub Date : 2023-05-17 , DOI: 10.1021/acsfoodscitech.3c00031
Sandip Nathani ₁ , C. N. Neeraja ₂ , R. M. Sundaram ₂ , Partha Roy ₁

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Zinc (Zn) and iron (Fe) are two essential micronutrients that help the body to maintain its normal physiology and metabolism. Rice is the most important staple crop because it nourishes approximately half of the world’s population. The present study was intended to screen a set of eight rice varieties for total grain Zn and Fe content along with their uptake in differentiated Caco-2 cells. The total grain Zn and Fe content, as well as their bioaccessibility, was determined using inductively coupled plasma mass spectrometry (ICP-MS) techniques. The Caco-2 cell-based fluorescence assays and ferritin synthesis assays were used to determine Zn and Fe uptake, respectively. The transcriptional expression of micronutrient transporters has been analyzed in Caco-2 cells in response to the uptake of Zn and Fe from in vitro digested rice samples. The findings of this study revealed a comparative analysis of two prominent micronutrient contents in eight different varieties of polished rice. DRRDhan 48 (4.93 ± 0.10 mg/kg), Zinco Rice (4.65 ± 0.01 mg/kg), and Protozin (4.43 ± 0.03 mg/kg) were shown to have low phytate content, and high grain Zn content (32.49 ± 2.8, 27.9 ± 1.8, 26.7 ± 1.2 mg/kg, respectively), as well as significantly greater Zn uptake in differentiated Caco-2 cells. The outcome of this study provides an easy and convenient approach for the screening of food items or cereals based on micronutrient uptake.

中文翻译：

基于锌和铁的总含量及其被 Caco-2 细胞摄取的八个生物强化水稻品种的筛选：体外细胞模型

锌 (Zn) 和铁 (Fe) 是两种必需的微量营养素，可帮助人体维持正常的生理机能和新陈代谢。水稻是最重要的主粮作物，因为它养活了世界上大约一半的人口。本研究旨在筛选一组八个水稻品种的总籽粒锌和铁含量及其在分化的 Caco-2 细胞中的吸收。使用电感耦合等离子体质谱 (ICP-MS) 技术测定了总谷物 Zn 和 Fe 含量及其生物可及性。基于 Caco-2 细胞的荧光测定和铁蛋白合成测定分别用于确定 Zn 和 Fe 摄取。已经在 Caco-2 细胞中分析了微量营养素转运蛋白的转录表达，以响应从体外消化的水稻样品中摄取 Zn 和 Fe。这项研究的结果揭示了八种不同精米中两种主要微量营养素含量的比较分析。DRRDhan 48 (4.93 ± 0.10 mg/kg)、Zinco Rice (4.65 ± 0.01 mg/kg) 和 Protozin (4.43 ± 0.03 mg/kg) 被证明具有低植酸盐含量和高谷物锌含量（32.49 ± 2.8，分别为 27.9 ± 1.8、26.7 ± 1.2 mg/kg），以及分化的 Caco-2 细胞中锌的摄取量显着增加。这项研究的结果为根据微量营养素摄取筛选食品或谷物提供了一种简单方便的方法。和高谷物锌含量（分别为 32.49 ± 2.8、27.9 ± 1.8、26.7 ± 1.2 mg/kg），以及分化的 Caco-2 细胞对锌的吸收显着增加。这项研究的结果为根据微量营养素摄取筛选食品或谷物提供了一种简单方便的方法。和高谷物锌含量（分别为 32.49 ± 2.8、27.9 ± 1.8、26.7 ± 1.2 mg/kg），以及分化的 Caco-2 细胞对锌的吸收显着增加。这项研究的结果为根据微量营养素摄取筛选食品或谷物提供了一种简单方便的方法。

更新日期：2023-05-17

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