Bioresource Technology ( IF 9.7 ) Pub Date : 2023-05-16 , DOI: 10.1016/j.biortech.2023.129186
Yaqi Zhang 1 , Yuzhe He 1 , Jingfei Huang 2 , Jiejing Chen 1 , Xiaoshan Jia 3 , Xingxing Peng 3
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Candida tropicalis PNY, a novel dimorphic strain with the capacity of simultaneous carbon, nitrogen and phosphorus removal in anaerobic and aerobic conditions, was isolated from activated sludge. Dimorphism of C. tropicalis PNY had effect on removing nitrogen and phosphorous and slightly affected COD removal under aerobic condition. Sample with high hypha formation rate (40 ± 5%) had more removal efficiencies of NH4+-N (50 mg/L) and PO43−-P (10 mg/L), which could achieve 82.19% and 97.53%, respectively. High hypha cells dosage exhibited good settleability and filamentous overgrowth was not observed. According to label-free quantitative proteomics assays. Up-regulated proteins involved in the mitogen-activated protein kinase (MAPK) pathway indicated the active growth and metabolism process of sample with high hypha formation rate (40 ± 5%). And proteins concerning about glutamate synthetase and SPX domain-contain protein explain for the nutrient removal mechanism including assimilation of ammonia and polyphosphates synthesis.
中文翻译:
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热带假丝酵母的二型性及其对脱氮除磷和污泥沉降性的影响
热带假丝酵母PNY 是一种新型双态菌株,具有在厌氧和好氧条件下同时去除碳、氮和磷的能力,是从活性污泥中分离出来的。C. tropicalis PNY的二态性对脱氮除磷有影响,对好氧条件下COD的去除影响较小。菌丝形成率高 (40 ± 5%) 的样品对 NH 4 + -N (50 mg/L) 和 PO 4 3−的去除效率更高-P (10 mg/L),分别达到82.19%和97.53%。高菌丝细胞剂量表现出良好的沉降性,并且未观察到丝状过度生长。根据无标记定量蛋白质组学分析。参与丝裂原活化蛋白激酶 (MAPK) 通路的上调蛋白表明具有高菌丝形成率 (40 ± 5%) 的样品的活跃生长和代谢过程。与谷氨酸合成酶有关的蛋白质和含有 SPX 结构域的蛋白质解释了包括氨同化和聚磷酸盐合成在内的营养去除机制。