This study evaluated periphyton biomass, nutrient content, and taxonomical composition in three nutrient-poor post-mining lakes in the Czech Republic. Two methods, microscopy and chemotaxonomy, were used to determine the taxonomical composition of autotrophs. Both methods identified diatoms, Chlorophyta, and Cyanobacteria as the dominant groups across the lakes. Considerable congruence of the taxonomical methods was found for diatoms and Chlorophyta, however results for Cyanobacteria showed poor correlation. The differences in periphyton features among the lakes were mostly explained by the lake age and trophy. Moreover, high amounts of overwintering biomass show that periphyton development is not established “de novo” each year but its current stage is a cumulative result of previous years. Beside the lake age and trophy, limnological characteristics such as Si or Mg²⁺ also affect periphyton taxonomical composition. No correlation of periphytic C:N:P molar ratios with lake water nitrogen and phosphorus, suggests role of additional process to the nutrient uptake, likely internal nutrient recycling in periphyton. These findings are essential in predicting further succession in the examined post-mining lakes and serve as a model for newly formed lakes. As more lakes will be formed within the post-mining recultivation in the short horizon, our study contributes to their successful management.

本研究评估了捷克共和国三个营养贫乏的采矿后湖泊的附生生物量、营养成分和分类组成。两种方法，显微镜和化学分类学，被用来确定自养生物的分类组成。这两种方法都将硅藻、绿藻和蓝藻确定为整个湖泊的主要群体。发现硅藻和绿藻的分类方法相当一致，但蓝藻的结果显示相关性较差。湖泊间附生生物特征的差异主要由湖泊年龄和营养物来解释。此外，大量越冬生物量表明附生生物的发育并未“从头开始”每一年，但它现在的阶段，都是前几年累积的结果。除了湖泊年龄和营养物之外，Si 或 Mg ²⁺等湖泊学特征也会影响附生生物分类学组成。附生植物 C:N:P 摩尔比与湖水氮和磷没有相关性，表明附加过程对养分吸收的作用，可能是附生生物的内部养分循环。这些发现对于预测所检查的采矿后湖泊的进一步演替至关重要，并可作为新形成湖泊的模型。随着短期内采矿后复垦中将形成更多的湖泊，我们的研究有助于它们的成功管理。