共价有机骨架-MnO2 纳米颗粒复合材料用于双硫醇的形状选择性传感,RSC Advances

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共价有机骨架-MnO2 纳米颗粒复合材料用于双硫醇的形状选择性传感
RSC Advances ( IF 3.9 ) Pub Date : 2023-05-16 , DOI: 10.1039/d3ra01540h
Yuping Cao ₁ , Jin Zhang ₁ , Jilu Yang ₁ , Wenwu Qin _{1,

2}

Affiliation

用于检测水或生物环境中的生物大分子的共价有机骨架 (COF) 通常具有挑战性。在这项工作中，复合材料 IEP-MnO ₂是通过将二氧化锰 (MnO ₂ ) 纳米晶体与荧光 COF (IEP) 结合而获得的，该材料是使用 2,4,6-三(4-氨基苯基)- s -合成的三嗪和 2,5-二甲氧基对苯二甲醛。通过添加不同大小的生物硫醇，如谷胱甘肽、半胱氨酸或同型半胱氨酸，IEP-MnO ₂的荧光发射光谱通过不同的机制发生变化（“开启”或“关闭”）。_{IEP-MnO 2}的荧光发射_{通过消除 MnO 2}和 IEP之间的 FRET（Förster 共振能量转移）效应，在存在 GSH 的情况下增加。令人惊讶的是，由于 Cys/Hcy 和 IEP 之间形成氢键，IEP-MnO ₂ + Cys/Hcy 的荧光猝灭可以通过光电子转移 (PET) 过程来解释，这赋予 IEP-MnO ₂在与其他 MnO ₂复合材料相比，区分 GSH 和 Cys/Hcy 的检测。因此，IEP-MnO ₂分别用于检测人全血和血清中的GSH和Cys。全血中 GSH 和人血清中 Cys 的检测限计算为 25.58 μM 和 4.43 μM，这表明 IEP-MnO₂可用于研究一些与 GSH 和 Cys 浓度相关的疾病。此外，该研究扩展了共价有机框架在荧光传感领域的应用。

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Covalent organic framework-MnO2 nanoparticle composites for shape-selective sensing of bithiols

Covalent organic frameworks (COFs) for detecting biological macromolecules in water or biological environments are generally challenging. In this work, a composite material IEP-MnO₂ is obtained by combining manganese dioxide (MnO₂) nanocrystals and a fluorescent COF (IEP), which is synthesized by using 2,4,6-tris(4-aminophenyl)-s-triazine and 2,5-dimethoxyterephthalaldehyde. By the addition of biothiols, such as glutathione, cysteine or homocysteine with different sizes, the fluorescence emission spectra of IEP-MnO₂ changed (“turn-on” or “turn-off”) via different mechanisms. The fluorescence emission of IEP-MnO₂ increased in the presence of GSH by the elimination of the FRET (Förster resonance energy transfer) effect between MnO₂ and IEP. Surprisingly, due to the formation of a hydrogen bond between Cys/Hcy and IEP, the fluorescence quenching for IEP-MnO₂ + Cys/Hcy may be explained via the photoelectron transfer (PET) process, which endows IEP-MnO₂ with specificity in distinguishing the detection of GSH and Cys/Hcy compared to other MnO₂ complex materials. Therefore, IEP-MnO₂ was used to detect GSH and Cys in human whole blood and serum, respectively. The limit of detection for GSH in whole blood and Cys in human serum was calculated to be 25.58 μM and 4.43 μM, which indicates that IEP-MnO₂ can be used to investigate some diseases related to GSH and Cys concentration. Moreover, the research expands the application of covalent organic frameworks in the fluorescence sensing field.

更新日期：2023-05-16

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