破译沙参麦冬汤治疗肺癌的关键成分及作用机制,BMC Complementary and Alternative Medicine

当前位置： X-MOL 学术 › BMC Complement. Altern. Med. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

破译沙参麦冬汤治疗肺癌的关键成分及作用机制
BMC Complementary and Alternative Medicine Pub Date : 2023-05-15 , DOI: 10.1186/s12906-023-03985-y
Jieqi Cai , Yupeng Chen , Kexin Wang , Yi Li , Jie Wu , Hailang Yu , Qingping Li , Qi Wu , Wei Meng , Handuo Wang , Aiping Lu , Mianbo Huang , Genxia Wei , Daogang Guan

肺癌是全球发病率和死亡率增长最快的恶性肿瘤。可用的临床治疗具有显着的副作用，因此需要确定治疗肺癌的替代方式。沙参麦冬汤(SMD)是临床上治疗肺癌的常用中药方剂。而SMD治疗肺癌的关键功能成分（KFC）和潜在机制仍不清楚。我们提出了一种新的综合药理学模型，该模型结合了一种新的节点重要性计算方法和贡献决策率 (CDR) 模型，以识别 SMD 的 KFC 并推断其在肺癌治疗中的机制。从我们提出的节点重要性检测方法中选择的丰富的有效基因本体 (GO) 术语可以覆盖 97.66% 的丰富的 GO 术语参考目标。通过计算关键功能网络中活跃组件的CDR，前82个组件覆盖了90.25%的网络信息，定义为肯德基。对 82 家肯德基进行了功能分析和实验验证。5-40 μM原儿茶酸、100-400 μM丹皮酚或咖啡酸对A549细胞的增殖具有显着抑制作用。结果表明，KFC通过靶向活跃于肺癌的PI3K-Akt、MAPK、SCLC和NSCLC信号通路中的Ras、AKT、IKK、Raf1、MEK和NF-κB，在肺癌的治疗中发挥重要的治疗作用. 本研究为中药方剂优化及二次开发提供方法学参考。本研究提出的策略可用于识别复杂网络中的关键化合物，为后续实验验证提供了可操作的测试范围，大大减少了实验工作量。

"点击查看英文标题和摘要"

Decoding the key compounds and mechanism of Shashen Maidong decoction in the treatment of lung cancer

Lung cancer is a malignant tumour with the fastest increase in morbidity and mortality around the world. The clinical treatments available have significant side effects, thus it is desirable to identify alternative modalities to treat lung cancer. Shashen Maidong decoction (SMD) is a commonly used traditional Chinese medicine (TCM) formula for treating lung cancer in the clinic. While the key functional components (KFC) and the underlying mechanisms of SMD treating lung cancer are still unclear. We propose a new integrated pharmacology model, which combines a novel node-importance calculation method and the contribution decision rate (CDR) model, to identify the KFC of SMD and to deduce their mechanisms in the treatment of lung cancer. The enriched effective Gene Ontology (GO) terms selected from our proposed node importance detection method could cover 97.66% of enriched GO terms of reference targets. After calculating CDR of active components in key functional network, the first 82 components covered 90.25% of the network information, which were defined as KFC. 82 KFC were subjected to functional analysis and experimental validation. 5–40 μM protocatechuic acid, 100–400 μM paeonol or caffeic acid exerted significant inhibitory activity on the proliferation of A549 cells. The results show that KFC play an important therapeutic role in the treatment of lung cancer by targeting Ras, AKT, IKK, Raf1, MEK, and NF-κB in the PI3K-Akt, MAPK, SCLC, and NSCLC signaling pathways active in lung cancer. This study provides a methodological reference for the optimization and secondary development of TCM formulas. The strategy proposed in this study can be used to identify key compounds in the complex network and provides an operable test range for subsequent experimental verification, which greatly reduces the experimental workload.

更新日期：2023-05-15

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南