肠道微生物群紊乱与便秘有关。本研究调查了脾虚便秘小鼠肠粘膜微生物群介导的微生物群-肠-脑轴和氧化应激。将昆明小鼠随机分为对照组(MC)和便秘组(MM)。采用番泻叶汤灌胃并控制饮食和饮水建立脾虚便秘模型。 MM组体重、脾胸腺指数、5-羟色胺(5-HT)、超氧化物歧化酶(SOD)含量显着低于MC组,血管活性肠肽(VIP)、丙二醛(MDA)含量显着低于MC组含量显着高于MC组。脾虚便秘小鼠肠粘膜细菌的α多样性没有变化,但β多样性发生了变化。与MC组相比,MM组Proteobacteria相对丰度呈上升趋势,Firmicutes/Bacteroidota(F/B)值呈下降趋势。两组之间的特征微生物群存在显着差异。 MM组中,短毛菌属、阿克曼氏菌属、副氏菌属、粪杆菌属、气单胞菌属、鞘氨醇杆菌属、放线杆菌属等致病菌富集。同时,微生物群与胃肠神经肽和氧化应激指标之间存在一定的关系。脾虚便秘小鼠肠粘膜细菌群落结构发生改变,其特点是F/B值降低、变形菌门富集。 微生物群-肠-脑轴可能对脾虚便秘很重要。
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Brain-bacteria-gut axis and oxidative stress mediated by intestinal mucosal microbiota might be an important mechanism for constipation in mice
Intestinal microbiota disorder was associated with constipation. This study investigated the microbiota–gut–brain axis and oxidative stress mediated by intestinal mucosal microbiota in mice with spleen deficiency constipation. The Kunming mice were randomly divided into the control (MC) group and the constipation (MM) group. The spleen deficiency constipation model was established by gavage with Folium sennae decoction and controlled diet and water intake. The body weight, spleen and thymus index, 5-Hydroxytryptamine (5-HT) and Superoxide Dismutase (SOD) content were significantly lower in the MM group than the MC group, the content of vasoactive intestinal peptide (VIP) and malondialdehyde (MDA) content were significantly higher than the MC group. The Alpha diversity of intestinal mucosal bacteria was not changed but beta diversity was changed in mice with spleen deficiency constipation. Compared to the MC group, the relative abundance of Proteobacteria was an upward trend and the Firmicutes/Bacteroidota (F/B) value was a downward trend in the MM group. There was a significant difference in the characteristic microbiota between the two groups. In the MM group, Brevinema, Akkermansia, Parasutterella, Faecalibaculum, Aeromonas, Sphingobium, Actinobacillus, and other pathogenic bacteria were enriched. Meanwhile, there was a certain relationship between the microbiota and gastrointestinal neuropeptide and oxidative stress indicators. The community structure of intestinal mucosal bacteria in mice with spleen deficiency constipation was changed, which was characterized by the reduction of F/B value and enrichment of Proteobacteria. Microbiota–gut–brain axis may be important for spleen deficiency constipation.