Microchimica Acta ( IF 5.3 ) Pub Date : 2023-05-15 , DOI: 10.1007/s00604-023-05793-0 Lixin Xu 1 , Shenglan Hu 1 , Dongmiao Qin 1 , Yusheng Wu 1 , Zhi Luo 1 , Biyang Deng 1
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This study aimed to design a sandwich electrochemiluminescence (ECL) immunosensor with double co-reaction accelerators for sensitively detecting squamous cell carcinoma antigen (SCCA). First, silver orthophosphate (Ag3PO4) nanoparticles were modified on the surface of EuPO4 nanowires to improve their poor dispersibility/solubility. At the same time, EuPO4 was used as a co-reaction accelerator to catalyze S2O82− to produce more intermediates (SO4•−), significantly enhancing the ECL signal of Ag3PO4. Ag nanoparticles (AgNP) modified on Ag3PO4@EuPO4 composite nanomaterials were used not only as linkers of luminescence groups and biomarkers but also as a co-reaction accelerator to effectively enhance ECL signal. The designed ECL immunosensor displayed several advantages, including good stability and reproducibility. Under the optimal conditions, its linear range in detecting SCCA was 0.0001–50 ng·mL−1, the detection limit was 25 fg·mL−1 (S/N = 3), the recovery was 96.6–100.4%, and the relative standard deviation was less than 4.8%. It was successfully applied to detect SCCA in human serum.
Graphical Abstract
中文翻译：
基于Ag3PO4@EuPO4-AgNP双共反应加速器电化学发光免疫传感器检测鳞状细胞癌抗原
本研究旨在设计一种具有双共反应加速器的夹心电化学发光（ECL）免疫传感器，用于灵敏检测鳞状细胞癌抗原（SCCA）。首先，在EuPO 4纳米线的表面修饰正磷酸银(Ag 3 PO 4 )纳米粒子，以改善其较差的分散性/溶解性。同时，EuPO 4用作共反应促进剂，催化S 2 O 8 2−生成更多中间体(SO 4 •− )，显着增强Ag 3 PO 4的ECL信号。Ag 3 PO 4 @EuPO 4复合纳米材料修饰的银纳米颗粒（AgNP）不仅可以用作发光基团和生物标志物的连接体，还可以作为共反应加速剂，有效增强ECL信号。设计的ECL免疫传感器具有多种优点，包括良好的稳定性和重现性。在最佳条件下，其检测SCCA的线性范围为0.0001～50 ng·mL -1，检出限为25 fg·mL -1 (S/N = 3)，回收率为96.6%～100.4%，相对标准偏差小于4.8%。成功应用于人血清中SCCA的检测。
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