We developed a biosynthetic route from m-nitroacetophenone (1a) to 3-amino-2-hydroxy acetophenone (1c) via 3-hydroxy amino acetophenone (1b) using a direct reaction method with three enzymes and supersaturated substrates. Through enzyme molar ratios, expression, and structure optimization, we obtained the optimal mutant with a biosynthetic yield of 580 mg/L.

中文翻译：

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多酶生物合成高效生产3-氨基-2-羟基苯乙酮

我们采用三种酶和过饱和底物的直接反应方法，开发了一种从间硝基苯乙酮 (1a) 通过 3-羟基氨基苯乙酮 (1b) 到 3-氨基-2-羟基苯乙酮 (1c) 的生物合成路线。通过酶摩尔比、表达和结构优化，我们获得了生物合成产量为580 mg/L的最佳突变体。