Isoform-Selective Fluorescent Labeling of 14-3-3σ by Acrylamide-Containing Fusicoccins,Chemistry - A European Journal

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Isoform-Selective Fluorescent Labeling of 14-3-3σ by Acrylamide-Containing Fusicoccins
Chemistry - A European Journal ( IF 3.9 ) Pub Date : 2023-05-12 , DOI: 10.1002/chem.202301059
Kenta Tanaka ₁ , Yoshiya Hatano ₁ , Junko Ohkanda _{1,

2}

Affiliation

14-3-3 isoforms and the elucidation of their biological roles: In vitro isoform-selective fluorescent labeling of 14-3-3σ was achieved by a fusicoccin derivative containing a fluorophore and a Cys-directed acrylamide group. The reaction proceeds upon ternary complex formation with mode-3 phosphopeptide, which possesses i+1 aliphatic residues.

中文翻译：

含丙烯酰胺的梭菌素对 14-3-3σ 进行异构体选择性荧光标记

14-3-3 同工型及其生物学作用的阐明：通过含有荧光团和 Cys 定向丙烯酰胺基团的梭菌素衍生物实现了 14-3-3σ 的体外同工型选择性荧光标记。该反应在与具有 i+1 个脂肪族残基的 3 型磷酸肽形成三元复合物后进行。

更新日期：2023-05-12

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