基于类氧化酶 CoFeMn 纳米酶的双模式纸生物传感平台用于即时检测谷胱甘肽,ACS Applied Nano Materials

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基于类氧化酶 CoFeMn 纳米酶的双模式纸生物传感平台用于即时检测谷胱甘肽
ACS Applied Nano Materials ( IF 5.3 ) Pub Date : 2023-05-11 , DOI: 10.1021/acsanm.3c01310
Hongyuan Shang ₁ , Xiaofei Zhang ₁ , Meili Ding ₁ , Aiping Zhang ₁ , Cheng Wang ₂

Affiliation

基于纸张的生物传感平台是一种用于检测各种生物标志物的即时 (POC) 分析工具包。尽管如此，其缺乏高灵敏度、选择性和多信号读出带来了很大的挑战。在此，我们介绍了一种有趣的基于多功能 CoFeMn 仿生氧化酶的比色温度双信号读出生物传感器。由于具有成本效益、不需要额外的检测器、双信号读出能力和更暗的信号变化，CoFeMn hydroxide 以 Mn 作为活性中心，通过简便的水热法创新地制造，构建了一个多功能信号标签，用于模拟结构氧化酶。这种策略允许 CoFeMn 氢氧化物表现出不寻常的氧化酶样活性，在没有 H 的情况下将 O _{2转化为·OH}₂氧₂并通过氧化 3,3',5,5'-四甲基联苯胺 (TMB) 产生比色温度双重信号。我们以谷胱甘肽（GSH）为分析剂，成功应用该生物传感器测定GSH，显示出快速、高特异性、灵敏度和准确性的优良分析性能。使用比色和温度测试条，这些工具包旨在可视化 GSH。得益于相对独立的信号转导通路，所构建的试纸POC装置显示出令人满意的耦合效率，并进一步用于监测肿瘤细胞中的GSH。本研究将有助于在纸基POC装置中合理设计基于模拟氧化酶的双响应纳米探针，探索更多纳米材料衍生的模拟氧化酶，

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Dual-Mode Paper Biosensing Platform Based on Oxidase-Like CoFeMn Nanozymes for Point-of-Care Detection of Glutathione

A paper-based biosensing platform is a point-of-care (POC) analysis toolkit for detection of various biomarkers. Nonetheless, its lack of high sensitivity, selectivity, and multi-signal readout poses a high degree of challenge. Herein, we introduce an interesting colorimetric-temperature dual-signal readout biosensor based on multifunctional CoFeMn biomimetic oxidase. Due to the cost-effectiveness, no additional detector requirement, dual-signal readout capacity, and darker signal change, CoFeMn hydroxide is innovatively fabricated by a facile hydrothermal method using Mn as an active center to construct a multifunctional signal tag for mimicking the structure of oxidase. This strategy allows CoFeMn hydroxide to exhibit an unusual oxidase-like activity, transforming O₂ into ·OH in the absence of H₂O₂ and resulting in the generation of a colorimetric temperature dual signal by oxidizing 3,3′,5,5′-tetramethylbenzidine (TMB). We used glutathione (GSH) as the analyst and successfully applied this biosensor to determine GSH, which showed excellent analytical performance of rapidity, high specificity, sensitivity, and accuracy. Employing the colorimetric and temperature test strips, these toolkits are designed to visualize GSH. Benefiting from a relatively independent signal transduction pathway, the constructed test strip POC devices display satisfactory coupling efficiency and are further employed to monitor GSH in tumor cells. This research will be conducive to the rational design of a mimetic oxidase-based dual-response nanoprobe in paper-based POC devices, the exploration of more nanomaterial-derived mimetic oxidase, and the extension of their applications in medical diagnosis.

更新日期：2023-05-11

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