Darobactin B Stabilises a Lateral-Closed Conformation of the BAM Complex in E. coli Cells,Angewandte Chemie International Edition

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Darobactin B Stabilises a Lateral-Closed Conformation of the BAM Complex in E. coli Cells
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2023-05-10 , DOI: 10.1002/anie.202218783
Samuel F Haysom ₁ , Jonathan Machin ₁ , James M Whitehouse ₁ , Jim E Horne ₁ , Katherine Fenn ₁ , Yue Ma _{2,

3,

4} , Hassane El Mkami ₅ , Nils Böhringer _{6,

7} , Till F Schäberle _{6,

7,

8} , Neil A Ranson ₁ , Sheena E Radford ₁ , Christos Pliotas _{2,

3,

4}

Affiliation

The darobactins are promising new antibiotics that inhibit the BAM complex, an essential membrane-protein foldase in Gram-negative bacteria. Here, we use EPR spectroscopy (PELDOR, or DEER) distance measurements to monitor the effect of darobactin B on BAM in E. coli cells, showing it stabilises BAM in a lateral gate closed conformational state. Our results provide the first direct evidence for conformational selection by a darobactin in the cellular context.

中文翻译：

Darobactin B 稳定大肠杆菌细胞中 BAM 复合物的侧闭合构象

darobactins 是有前途的新型抗生素，可抑制 BAM 复合物，BAM 复合物是革兰氏阴性菌中必不可少的膜蛋白折叠酶。在这里，我们使用 EPR 波谱（PELDOR 或 DEER）距离测量来监测 darobactin B 对大肠杆菌细胞中 BAM 的影响，表明它在侧门关闭构象状态下稳定 BAM。我们的结果为 darobactin 在细胞环境中的构象选择提供了第一个直接证据。

更新日期：2023-05-10

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