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Autonomously Assembled Living Capsules by Microbial Coculture for Enhanced Bacteriotherapy of Inflammatory Bowel Disease
Nano Letters ( IF 9.6 ) Pub Date : 2023-05-09 , DOI: 10.1021/acs.nanolett.3c00657 Ji-Yan Qiao 1 , Run-Yao Zeng 1 , Qi-Wen Chen 1 , Diwei Zheng 1 , Pei-Ling Hao 1 , Xin-Hua Liu 1 , Faqiong Zhao 2 , Xian-Zheng Zhang 1
Nano Letters ( IF 9.6 ) Pub Date : 2023-05-09 , DOI: 10.1021/acs.nanolett.3c00657 Ji-Yan Qiao 1 , Run-Yao Zeng 1 , Qi-Wen Chen 1 , Diwei Zheng 1 , Pei-Ling Hao 1 , Xin-Hua Liu 1 , Faqiong Zhao 2 , Xian-Zheng Zhang 1
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Microorganism-mediated self-assembling of living formulations holds great promise for disease therapy. Here, we constructed a prebiotic–probiotic living capsule (PPLC) by coculturing probiotics (EcN) with Gluconacetobacter xylinus (G. xylinus) in a prebiotic-containing fermentation broth. Through shaking the culture, G. xylinus secretes cellulose fibrils that can spontaneously encapsulate EcN to form microcapsules under shear forces. Additionally, the prebiotic present in the fermentation broth is incorporated into the bacterial cellulose network through van der Waals forces and hydrogen bonding. Afterward, the microcapsules were transferred to a selective LB medium, which facilitated the colonization of dense probiotic colonies within them. The in vivo study demonstrated that PPLC-containing dense colonies of EcN can antagonize intestinal pathogens and restore microbiota homeostasis by showing excellent therapeutic performance in treating enteritis mice. The in situ self-assembly of probiotics and prebiotics-based living materials provides a promising platform for the treatment of inflammatory bowel disease.
更新日期：2023-05-09
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