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Selective Peptide Binders to the Perfluorinated Sulfonic Acid Ionomer Nafion
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2023-05-04 , DOI: 10.1002/adfm.202214932 Dimitry Schmidt 1 , Patrizia Gartner 2 , Ivan Berezkin 1 , Jens Rudat 3 , Maximilian Bilger 3 , Tom Grünert 3 , Nadine Zimmerer 4 , Philipp Quarz 4 , Philip Scharfer 4 , Julian Brückel 5 , André P. Jung 5 , Pooja Singh 6 , Pooja Pooja 6 , Benno Meier 6 , Mareen Stahlberger 1 , Wilhelm Schabel 4 , Stefan Bräse 5 , Gisela Lanza 2 , Alexander Nesterov‐Mueller 1
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2023-05-04 , DOI: 10.1002/adfm.202214932 Dimitry Schmidt 1 , Patrizia Gartner 2 , Ivan Berezkin 1 , Jens Rudat 3 , Maximilian Bilger 3 , Tom Grünert 3 , Nadine Zimmerer 4 , Philipp Quarz 4 , Philip Scharfer 4 , Julian Brückel 5 , André P. Jung 5 , Pooja Singh 6 , Pooja Pooja 6 , Benno Meier 6 , Mareen Stahlberger 1 , Wilhelm Schabel 4 , Stefan Bräse 5 , Gisela Lanza 2 , Alexander Nesterov‐Mueller 1
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Fuel cells used for transport applications hold polymer membranes as a key element. Their efficiency can be significantly increased if structured ion channels are implemented at the molecular level into the proton-conducting membrane. New functional molecules with selective affinity for ionomers are needed to obtain such a membrane design. This study presents a method to screen for selective peptide binders to perfluorinated sulfonic acid ionomers, e.g., Nafion using ultra-high density peptide arrays with a spot size of up to 30 µm. First, the ionomer dispersion is incubated with the peptide chip containing 56014 randomly chosen 6-mer peptides. Afterward, the peptide chip is washed. The peptide WIWHCW with the helix structure is identified as a selective binder to Nafion. The invariant amino acids responsible for binding are also determined using a peptide chip approach. The specific binding pocket of WIWHCW is formed by histidine and tryptophan. Its dissociation constant to the ionomer is measured at ≈140 µM.
中文翻译：
全氟磺酸离聚物 Nafion 的选择性肽结合剂
用于运输应用的燃料电池将聚合物膜作为关键元件。如果在分子水平上将结构化离子通道植入质子传导膜中，则它们的效率可以显着提高。需要对离聚物具有选择性亲和力的新功能分子来获得这样的膜设计。本研究提出了一种使用斑点尺寸高达 30 µm 的超高密度肽阵列筛选全氟磺酸离聚物（例如 Nafion）的选择性肽结合剂的方法。首先，将离聚物分散体与含有 56014 个随机选择的 6 聚体肽的肽芯片一起孵育。然后，洗涤肽芯片。具有螺旋结构的肽 WIWHCW 被鉴定为 Nafion 的选择性结合剂。负责结合的不变氨基酸也可以使用肽芯片方法来确定。 WIWHCW 的特异性结合袋由组氨酸和色氨酸形成。测得其与离聚物的解离常数约为 140 µM。
更新日期：2023-05-04
中文翻译：
全氟磺酸离聚物 Nafion 的选择性肽结合剂
用于运输应用的燃料电池将聚合物膜作为关键元件。如果在分子水平上将结构化离子通道植入质子传导膜中，则它们的效率可以显着提高。需要对离聚物具有选择性亲和力的新功能分子来获得这样的膜设计。本研究提出了一种使用斑点尺寸高达 30 µm 的超高密度肽阵列筛选全氟磺酸离聚物（例如 Nafion）的选择性肽结合剂的方法。首先，将离聚物分散体与含有 56014 个随机选择的 6 聚体肽的肽芯片一起孵育。然后，洗涤肽芯片。具有螺旋结构的肽 WIWHCW 被鉴定为 Nafion 的选择性结合剂。负责结合的不变氨基酸也可以使用肽芯片方法来确定。 WIWHCW 的特异性结合袋由组氨酸和色氨酸形成。测得其与离聚物的解离常数约为 140 µM。
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