ABSTRACT

DP915635 maize was genetically modified (GM) to express the IPD079Ea protein for corn rootworm (Diabrotica spp.) control. DP915635 maize also expresses the phosphinothricin acetyltransferase (PAT) protein for tolerance to glufosinate herbicide and the phosphomannose isomerase (PMI) protein that was used as a selectable marker. A field study was conducted at ten sites in the United States and Canada during the 2019 growing season. Of the 11 agronomic endpoints that were evaluated, two of them (early stand count and days to flowering) were statistically significant compared with the control maize based on unadjusted p-values; however, these differences were not significant after FDR-adjustment of p-values. Composition analytes from DP915635 maize grain and forage (proximates, fiber, minerals, amino acids, fatty acids, vitamins, anti-nutrients, and secondary metabolites) were compared to non-GM near-isoline control maize (control maize) and non-GM commercial maize (reference maize). Statistically significant differences were observed for 7 of the 79 compositional analytes (16:1 palmitoleic acid, 18:0 stearic acid, 18:1 oleic acid, 18:2 linoleic acid, 24:0 lignoceric acid, methionine, and α-tocopherol); however, these differences were not significant after FDR-adjustment. Additionally, all of the values for composition analytes fell within the range of natural variation established from the in-study reference range, literature range, and/or tolerance interval. These results demonstrate that DP915635 is agronomically and compositionally comparable to non-GM maize represented by non-GM near-isoline control maize and non-GM commercial maize.

 抽象的

DP915635 玉米经过基因改造 (GM)，表达 IPD079Ea 蛋白，用于控制玉米根虫 (Diabrotica spp.)。 DP915635 玉米还表达草丁膦乙酰转移酶 (PAT) 蛋白（用于耐受草铵膦除草剂）和磷酸甘露糖异构酶 (PMI) 蛋白（用作选择标记）。 2019 年生长季节期间，我们在美国和加拿大的 10 个地点进行了实地研究。在评估的 11 个农艺终点中，根据未调整的 p 值，其中两个（早期林分数和开花天数）与对照玉米相比具有统计显着性；然而，在对 p 值进行 FDR 调整后，这些差异并不显着。将 DP915635 玉米籽粒和饲料的成分分析物（原品、纤维、矿物质、氨基酸、脂肪酸、维生素、抗营养物和次生代谢物）与非转基因近等系对照玉米（对照玉米）和非转基因进行比较商品玉米（参考玉米）。 79 种成分分析物中的 7 种（16:1 棕榈油酸、18:0 硬脂酸、18:1 油酸、18:2 亚油酸、24:0 木蜡酸、蛋氨酸和 α-生育酚）观察到显着的统计学差异;然而，这些差异在 FDR 调整后并不显着。此外，成分分析物的所有值均落在根据研究中参考范围、文献范围和/或公差区间建立的自然变异范围内。这些结果证明DP915635在农艺学和组成上与以非转基因近等系对照玉米和非转基因商业玉米为代表的非转基因玉米相当。