Engineering Glucose Dehydrogenase to Favor Totally Synthetic Biomimetic Cofactors Containing Carboxyl Group,ChemBioChem

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Engineering Glucose Dehydrogenase to Favor Totally Synthetic Biomimetic Cofactors Containing Carboxyl Group
ChemBioChem ( IF 2.6 ) Pub Date : 2023-05-03 , DOI: 10.1002/cbic.202300066
Jieyu Zhou ₁ , Xiangyuan Gu ₁ , Yichun Zhu ₁ , Zhenyan Tao ₂ , Ye Ni ₁

Affiliation

Glucose dehydrogenase from Sulfolobus solfataricus (SsGDH) was engineered to favor a synthesized unnatural cofactor 3-carbamoyl-1-(4-carboxybenzyl) pyridin-1-ium (BANA⁺). Mutation hotspots E44 and E114 were identified by in situ ligand minimization program. Double mutant E44D/E114 L with 7.7-fold increased activity and 10.6-fold increased catalytic efficiency toward BANA⁺ was obtained.

中文翻译：

工程化葡萄糖脱氢酶以支持含有羧基的全合成仿生辅因子

来自硫磺矿硫化叶菌( Ss GDH)的葡萄糖脱氢酶经过改造，有利于合成非天然辅因子 3-氨基甲酰基-1-(4-羧基苄基)吡啶-1-ium (BANA ⁺ )。通过原位配体最小化程序鉴定了突变热点E44和E114 。获得了双突变体E44D/E114 L，其对BANA ^{+ 的}活性增加了7.7倍，催化效率增加了10.6倍。

更新日期：2023-05-03

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