An AND-gate bioluminescent probe for precise tumor imaging,Chemical Science

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An AND-gate bioluminescent probe for precise tumor imaging
Chemical Science ( IF 7.6 ) Pub Date : 2023-05-03 , DOI: 10.1039/d3sc00556a
Chenchen Wang ₁ , Yajian Hong ₁ , Ling Dong ₂ , Hu Cheng ₁ , Duo Jin ₁ , Ronghua Zhao ₃ , Zian Yu ₁ , Yue Yuan ₁

Affiliation

Sensitivity and specificity are two indispensable requirements to ensure diagnostic accuracy. Dual-locked probes with “AND-gate” logic theory have emerged as a powerful tool to enhance imaging specificity, avoid “false positive” results, and realize correlation analysis. In addition, bioluminescence imaging (BLI) is an excitation-free optical modality with high sensitivity and low background and can thus be combined with a dual-locked strategy for precise disease imaging. Here, we developed a novel AND-gate bioluminescent probe, FK-Luc-BH, which is capable of responding to two different tumor biomarkers (cathepsin L and ClO⁻). The good specificity of FK-Luc-BH was proven, as an obvious BL signal could only be observed in the solution containing both cathepsin L (CTSL) and ClO⁻. 4T1-fLuc cells and tumors treated with FK-Luc-BH exhibited significantly higher BL signals than those treated with unresponsive control compound Ac-Luc-EA or cotreated with FK-Luc-BH and a ClO⁻ scavenger/cathepsin inhibitor, demonstrating the ability of FK-Luc-BH to precisely recognize tumors in which CTSL and ClO⁻ coexist.

中文翻译：

用于精确肿瘤成像的与门生物发光探针

灵敏度和特异度是保证诊断准确性不可缺少的两个要求。采用“与门”逻辑理论的双锁探针已成为增强成像特异性、避免“假阳性”结果和实现相关分析的有力工具。此外，生物发光成像 (BLI) 是一种无激发光学模态，具有高灵敏度和低背景，因此可以与双锁定策略相结合以进行精确的疾病成像。在这里，我们开发了一种新型与门生物发光探针FK-Luc-BH，它能够响应两种不同的肿瘤生物标志物（组织蛋白酶 L 和 ClO ^-）。FK-Luc-BH良好的特异性^{已被证实，因为只有在同时含有组织蛋白酶 L (CTSL) 和 ClO -}的溶液中才能观察到明显的 BL 信号。用FK-Luc-BH处理的 4T1-fLuc 细胞和肿瘤表现出比用无反应对照化合物Ac-Luc-EA处理或用FK-Luc-BH和 ClO ^-清除剂/组织蛋白酶抑制剂共同处理的细胞和肿瘤显着更高的 BL 信号，证明了这种能力FK -Luc-BH精确识别 CTSL 和 ClO ^-共存的肿瘤。

更新日期：2023-05-03

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