Journal of Materials Science: Materials in Medicine ( IF 4.2 ) Pub Date : 2023-04-28 , DOI: 10.1007/s10856-023-06717-3 Bárbara Araújo Dos Reis 1 , Natalia Da Ponte Leguizamón 1 , Yumi Chokyu Del Rey 2 , Leandro Fernandes 3 , Cássio do Nascimento 3 , Luis Geraldo Vaz 1, 3
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High implant survival rates have been achieved in recent decades due to continual modifications in implant design and surface topography, however there is still an ongoing quest to control peri-implant bone loss. The objective of this work was to develop Ti-35Nb-7Zr-5Ta (TNZT) alloys, perform physicochemical and morphological characterization of their surface modified by electrolytic oxidative plasma technique with ions related to osseointegration and lastly evaluate bacterial colonization in vitro. Three groups were evaluated: C group (polished TNZT), CaP group (sodium β glycerophosphate + calcium acetate) and Mg group (magnesium acetate). Before and after anodizing the surfaces, physicochemical and morphological analyses were performed: scanning electron microscopy with field emission gun (FEG-SEM), energy dispersion spectroscopy (EDS), X-ray diffraction (DRX), wettability (goniometer) and roughness (rugometer). Controlled and treated specimens were contaminated with unstimulated saliva collected from 10 healthy volunteers. Then, biofilm samples were collected and up to 35 microbial species, including commensal and pathogenic microorganisms, were identified and quantified by the Checkerboard DNA-DNA Hybridization method. The CaP group modified the surface morphology in the form of pores, while the Mg group modified it in the form of flakes. The contact angle was significantly smaller in the CaP group. The average roughness was higher in the CaP and Mg groups. A smaller total amount of bacteria was identified in the Mg group and relevant differences were found in the microbial profile associated with different surface treatments. Therefore, considering the microbiological profile and for the prevention of peri-implantitis, the Mg group presented more satisfactory and encouraging results for the manufacture of dental implants
Graphical Abstract
中文翻译：
细菌对含有钙、磷酸盐和镁的 Ti-35Nb-7Zr-5Ta 合金的反应
由于种植体设计和表面形貌的不断改进，近几十年来种植体的高存活率已经实现，但是控制种植体周围骨丢失的问题仍在继续。这项工作的目的是开发 Ti-35Nb-7Zr-5Ta (TNZT) 合金，通过电解氧化等离子体技术用与骨结合相关的离子对其表面进行物理化学和形态学表征，最后评估细菌在体外的定殖。评估了三组：C 组（抛光 TNZT）、CaP 组（β 甘油磷酸钠 + 醋酸钙）和 Mg 组（醋酸镁）。在阳极氧化表面之前和之后，进行了物理化学和形态分析：场发射枪扫描电子显微镜 (FEG-SEM)、能量色散光谱 (EDS)、X 射线衍射 (DRX)、润湿性（测角仪）和粗糙度（粗糙度计）。从 10 名健康志愿者收集的未受刺激的唾液污染了受控和处理过的标本。然后，收集了生物膜样本，并通过该方法鉴定和量化了多达 35 种微生物，包括共生微生物和病原微生物。棋盘 DNA-DNA 杂交方法。CaP基团以孔隙形式修饰表面形貌，而Mg基团以薄片状修饰。CaP 组的接触角明显更小。CaP 和 Mg 组的平均粗糙度较高。在 Mg 组中发现了较少的细菌总量，并且在与不同表面处理相关的微生物特征中发现了相关差异。因此，考虑到微生物特征和种植体周围炎的预防，Mg 组在牙种植体的制造中呈现出更令人满意和令人鼓舞的结果
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