Available knowledge about the exact impact of plant growth on the properties and functioning of no-till soils is somewhat limited, especially in tropical and subtropical regions. The starting hypothesis for this work was that long-term active plant biomass Input-APBI (aboveground plant shoot biomass) would improve pore system functioning in surface and subsurface soil layers by playing different, complementary roles in a previously degraded subtropical Acrisol. The hypothesis was checked by examining the results of a 34-yr field experiment involving five different cropping systems, namely: bare soil (BS), perennial pasture (PAST), oat/maize (O/M), oat + vetch/maize (O + V/M) and oat + vetch/ maize + cowpea (O + V/M + C). The soil was supplied with APBIs varying from 0.13 to 1.48 kg dry matter ha⁻¹ yr⁻¹. The APBIs for each cropping system were very low (BS), low (O/M), medium (O + V/M), high (O + V/M + C), and very high (PAST). The surface (0–5 cm) and subsurface (5–15 cm) soil layer were analyzed for static and dynamic properties of the pore system including bulk density; total, macro, and microporosity; the ability of the system to conduct air and water; continuity in soil pores; and plant-available water capacity. Micromorphological images of the soil revealed a complex pore network whose structure and functioning were both improved by the action of plants. In the surface soil layer, very high APBIs from pasture and high inputs from O + V/M + C increased total porosity by 11 and 14 %, respectively; pore continuity (N_cont) by 11 and 40 %, respectively; and microporosity by 31 and 23 %, respectively —all relative to bare soil. In the subsurface soil layer, pasture, and O + V/M + C decreased N_cont by 44 and 40 %, respectively, but increased water permeability (k_w) by a factor of 6.5 and 7, respectively. In addition, very high APBIs increased continuity and permeability to air in macropores relative to BS. Organized large macropores in the subsurface soil layer efficiently conducted water and air from the soil surface and acted as bridges between the surface and subsurface soil layer. Overall, our findings suggest that APBIs helped develop a pore system with differential properties and complementary functions that influenced water infiltration and air fluxes in the surface soil layer, and water availability to crops in the subsurface soil layer, through long-term self-organization in the system. Future research with a view to understanding the influence of species richness and roots on physical quality in no-till soils is recommended.