Cytosolic protein delivery using ultrasound-guided vaporization of perfluorocarbon nano-droplets,Biotechnology Journal

当前位置： X-MOL 学术 › Biotechnol. J. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Cytosolic protein delivery using ultrasound-guided vaporization of perfluorocarbon nano-droplets
Biotechnology Journal ( IF 3.2 ) Pub Date : 2023-04-25 , DOI: 10.1002/biot.202300018
Satoshi Yamaguchi ₁ , Miho Kisaka ₁ , Kotaro Higashi ₁ , Ayumu Ishijima ₂ , Takashi Azuma ₃ , Keiichi Nakagawa _{2,

4} , Yoshikazu Shibasaki ₅ , Ichiro Sakuma _{2,

4,

6} , Akimitsu Okamoto ₁

Affiliation

Ultrasound-guided protein delivery is promising for site-specific control of cellular functions in the deep interior of the body in a noninvasive manner. Herein, we propose a method for cytosolic protein delivery based on ultrasound-guided intracellular vaporization of perfluorocarbon nano-droplets. The nano-droplets were conjugated with cargo proteins through a bio-reductively cleavable linker and introduced into living cells via antibody-mediated binding to a cell-surface receptor, which gets internalized through endocytosis. After the cells were exposed to ultrasound for endosomal escape of proteins, the ultrasound-responsive cytosolic release of a cargo enzyme was confirmed by visualizing the hydrolysis of the fluorogenic substrate using confocal microscopy. Moreover, a significant decrease in cell viability was achieved via the release of a cytotoxic protein in response to ultrasound treatment. The results of this study provide the proof of a principle that protein-conjugated nano-droplets can be used as carriers in ultrasound-guided cytosolic delivery of proteins.

中文翻译：

使用超声引导全氟化碳纳米液滴汽化的胞浆蛋白递送

超声引导的蛋白质递送有望以非侵入性方式对身体深层的细胞功能进行位点特异性控制。在此，我们提出了一种基于超声引导全氟化碳纳米液滴的细胞内汽化的胞浆蛋白递送方法。纳米液滴通过生物还原可裂解接头与货物蛋白结合，并通过抗体介导的与细胞表面受体的结合引入活细胞，细胞表面受体通过内吞作用内化。将细胞暴露于超声波以进行蛋白质的内体逃逸后，通过使用共焦显微镜观察荧光底物的水解来确认货物酶的超声波响应胞质释放。而且，通过响应超声波处理释放细胞毒性蛋白，细胞活力显着降低。这项研究的结果证明了蛋白质缀合的纳米液滴可以用作超声引导的蛋白质胞质递送的载体的原理。

更新日期：2023-04-25

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南