<br>使用 HKCL-1M（一种高选择性和灵敏的荧光探针）原位观察心磷脂,Journal of the American Chemical Society

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使用 HKCL-1M（一种高选择性和灵敏的荧光探针）原位观察心磷脂
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2023-04-27 , DOI: 10.1021/jacs.3c00243
Wei Wang ₁ , Nai-Kei Wong ₂ , Siu-Lun Bok ₃ , You Xu ₄ , Yang Guo ₅ , Lu Xu ₆ , Meiling Zuo ₆ , Claudette M St Croix ₇ , Gaowei Mao _{8,

9} , Alexandr Kapralov _{8,

9} , Hülya Bayir _{9,

10} , Valerian E Kagan _{8,

9} , Dan Yang _{3,

6,

11}

Affiliation

Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communication, Institute of Photonics Technology, Jinan University, Guangzhou 510632, China.
Clinical Pharmacology Section, Department of Pharmacology, Shantou University Medical College, Shantou 515041, China.
Morningside Laboratory for Chemical Biology, Department of Chemistry, The University of Hong Kong, Hong Kong 999077, China.
Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou 310024, China.
Qingdao Institute for Theoretical and Computational Sciences, Institute of Frontier and Interdisciplinary Science, Shandong University, Qingdao 266237, China.
School of Life Sciences, Westlake University, Hangzhou 310024, China.
Department of Cell Biology, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, United States.
Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, United States.
Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States.
Department of Critical Care Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, United States.
Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou 310024, China.

可靠地探测动态细胞环境中的心磷脂 (CL) 含量，为了解线粒体相关疾病（包括癌症、神经退行性疾病和糖尿病）带来了重大挑战和巨大机遇。在完整的呼吸细胞中，由于磷脂之间的结构相似性和线粒体内膜的区室隔离性，CL 检测的选择性和灵敏度在技术上要求很高。在这里，我们报告了一种用于原位检测 CL 的新型“开启”荧光探针HKCL-1M 。 HKCL-1M通过特定的非共价相互作用对 CL 显示出出色的灵敏度和选择性。在活细胞成像中，其水解产物HKCL-1有效地保留在完整细胞中，不受线粒体膜电位 (Δψ _m ) 的影响。该探针与线粒体牢固地共定位，并且优于 10- N-壬基吖啶橙 (NAO) 和 Δψ _m依赖性染料，具有优异的光稳定性和可忽略不计的光毒性。因此，我们的工作通过高效可靠的 CL 原位可视化为研究线粒体生物学开辟了新的机会。

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更新日期：2023-04-27

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