<br>Neuromedin B 兴奋中央外侧杏仁核神经元并减少心血管输出和恐惧增强惊吓,Journal of Cellular Physiology

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Neuromedin B 兴奋中央外侧杏仁核神经元并减少心血管输出和恐惧增强惊吓
Journal of Cellular Physiology ( IF 4.5 ) Pub Date : 2023-04-26 , DOI: 10.1002/jcp.31020
Cody A Boyle ₁ , Saobo Lei ₁

Affiliation

神经调节素 B (NMB) 和胃泌素释放肽 (GRP) 是铃蟾肽家族中的两种哺乳动物类似物，发挥多种作用，包括情绪处理、食欲行为、认知和肿瘤生长。铃蟾肽样肽与三种受体相互作用：NMB 偏好的铃蟾肽 1 (BB1) 受体、GRP 偏好的铃蟾肽 2 (BB2) 受体和孤儿铃蟾肽 3 (BB3) 受体。然而，将铃蟾肽注射到中央杏仁核可降低饱腹感并调节血压，但其潜在的细胞和分子机制尚未确定。由于施用铃蟾肽会诱导表达 BB1 受体的中央杏仁核 (CeL) 外侧核中 Fos 的表达，因此我们使用体外和体内方法探讨了 NMB 对 CeL 神经元的影响。我们发现，BB1 受体的激活通过抑制内向整流 K ⁺ (Kir) 通道增加了 CeL 神经元记录的动作电位放电频率。磷脂酶 Cβ 和蛋白激酶 C 的活性是必需的，而细胞内 Ca ²⁺释放对于 BB1 受体引起的神经元兴奋性增强是不必要的。将 NMB 直接应用于 CeA 可以降低血压和心率，并显着减少恐惧增强惊吓。我们可以提供一种细胞和分子机制，使铃蟾肽样肽调节杏仁核中的焦虑和恐惧反应。

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Neuromedin B excites central lateral amygdala neurons and reduces cardiovascular output and fear-potentiated startle

Neuromedin B (NMB) and gastrin-releasing peptide (GRP) are the two mammalian analogs in the bombesin peptide family that exert a variety of actions including emotional processing, appetitive behaviors, cognition, and tumor growth. The bombesin-like peptides interact with three receptors: the NMB-preferring bombesin 1 (BB1) receptors, the GRP-preferring bombesin 2 (BB2) receptors and the orphan bombesin 3 (BB3) receptors. Whereas, injection of bombesin into the central amygdala reduces satiety and modulates blood pressure, the underlying cellular and molecular mechanisms have not been determined. As administration of bombesin induces the expression of Fos in the lateral nucleus of the central amygdala (CeL) which expresses BB1 receptors, we probed the effects of NMB on CeL neurons using in vitro and in vivo approaches. We showed that activation of the BB1 receptors increased action potential firing frequency recorded from CeL neurons via inhibition of the inwardly rectifying K⁺ (Kir) channels. Activities of phospholipase Cβ and protein kinase C were required, whereas intracellular Ca²⁺ release was unnecessary for BB1 receptor-elicited potentiation of neuronal excitability. Application of NMB directly into the CeA reduced blood pressure and heart rate and significantly reduced fear-potentiated startle. We may provide a cellular and molecular mechanism whereby bombesin-like peptides modulate anxiety and fear responses in the amygdala.

更新日期：2023-04-26

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