Electrochemiluminescence Immunosensors Based on ECL-RET Triggering between Mn SANE/PEI-Luminol and PtCu/h-MPF for Ultrasensitive Detection of CEA,Analytical Chemistry

当前位置： X-MOL 学术 › Anal. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Electrochemiluminescence Immunosensors Based on ECL-RET Triggering between Mn SANE/PEI-Luminol and PtCu/h-MPF for Ultrasensitive Detection of CEA
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-04-25 , DOI: 10.1021/acs.analchem.2c04397
Wenjing Lai ₁ , Jiajia Li ₁ , Mingzhe Jiang ₁ , Pengli Li ₁ , Min Wang ₁ , Chaoyun Ma ₁ , Chulei Zhao ₁ , Yu Qi ₁ , Chenglin Hong ₁

Affiliation

In this paper, a novel donor–acceptor pair was creatively proposed based on the principle of electrochemiluminescence resonance energy transfer (ECL-RET): luminol immobilized on polyethyleneimine (PEI)-functionalized manganese-based single-atom nanozymes (Mn SANE/PEI-luminol, donor) and a PtCu-grafted hollow metal polydopamine framework (PtCu/h-MPF, acceptor). A quenched ECL immunosensor was constructed for the ultrasensitive analysis of carcinoembryonic antigen (CEA). Mn SANE, as an efficient novel coreaction accelerator with the outstanding performance of significantly activating H₂O₂ to produce large amounts of ROS, was further modified by the coreactant PEI, which efficiently immobilized luminol to form a self-enhanced emitter. As a result, the electron transport distance was effectively shortened, the energy loss was reduced, and luminol achieved a high ECL efficiency. More importantly, PtCu-grafted h-MPF (PtCu/h-MPF) was proposed as a novel quencher. The UV–vis spectra of PtCu/h-MPF partially overlap with the ECL spectra of Mn SANE/PEI-luminol, which can effectively trigger the ECL-RET behavior between the donor and the acceptor. The multiple quenching effect on Mn SANE/PEI-luminol was achieved, which significantly improved the sensitivity of the immunosensor. The prepared immunosensor exhibited good linearity in the concentration range of 10^–5 to 80 ng/mL. The results indicate that this work provides a new method for the early detection of CEA in clinical diagnosis.

中文翻译：

基于 Mn SANE/PEI-Luminol 和 PtCu/h-MPF 之间 ECL-RET 触发的电化学发光免疫传感器用于 CEA 的超灵敏检测

本文基于电化学发光共振能量转移（ECL-RET）原理，创造性地提出了一种新型供体-受体对：固定在聚乙烯亚胺（PEI）上的鲁米诺功能化锰基单原子纳米酶（Mn SANE/PEI-鲁米诺，供体）和 PtCu 接枝的空心金属聚多巴胺骨架（PtCu/h-MPF，受体）。构建了淬灭的 ECL 免疫传感器，用于癌胚抗原 (CEA) 的超灵敏分析。Mn SANE，作为一种高效的新型共反应促进剂，具有显着活化H ₂ O _{2的突出性能}为了产生大量的 ROS，被共反应物 PEI 进一步修饰，它有效地固定了鲁米诺以形成自增强发射体。结果，有效缩短了电子传输距离，减少了能量损失，鲁米诺实现了高ECL效率。更重要的是，PtCu 接枝的 h-MPF (PtCu/h-MPF) 被提议作为一种新型猝灭剂。PtCu/h-MPF 的紫外-可见光谱与 Mn SANE/PEI-鲁米诺的 ECL 光谱部分重叠，可以有效触发供体和受体之间的 ECL-RET 行为。实现了对Mn SANE/PEI-鲁米诺的多重猝灭效应，显着提高了免疫传感器的灵敏度。^{制备的免疫传感器在10-5}的浓度范围内表现出良好的线性至 80 纳克/毫升。结果表明，该工作为临床诊断中CEA的早期检测提供了一种新方法。

更新日期：2023-04-25

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南