Development of sensitive, accurate yet simplified fluorescence sensor array is crucial for rapid, multi-bacterial, on-site sensing applications. A fluorescence sensor array based on a multiplexing fluorescence probe (MFP, excitations/emissions with 278 nm/558 nm, 290 nm/364 nm, 362 nm/592 and 616 nm) was constructed. The positive MFP equipped with rich quinoline and pyridine groups can combine with the negative charge groups and electron-rich groups of bacterial surfaces, which causes the symbolic signal response in the sensing channels (four emissions and three excitations). The sensor array displayed sensitive response to bacteria even at a lower level (10² cfu mL⁻¹) and high identification accuracy (100%) for drug-resistant bacteria, inactivated bacteria, and blends of bacteria. Furthermore, the developed sensor array demonstrated a high-throughput bacterial identification (92.1% accuracy) in real samples (pork and milk) with high sensitivity, rapid response, easy-to-operate, and independence of specific recognition elements, which would brighten the development of fluorescence sensor array.

开发灵敏、准确但简化的荧光传感器阵列对于快速、多细菌、现场传感应用至关重要。构建了基于多路复用荧光探针（MFP，激发/发射波长为 278 nm/558 nm、290 nm/364 nm、362 nm/592 和 616 nm）的荧光传感器阵列。富含喹啉和吡啶基团的正MFP可以与细菌表面的负电荷基团和富电子基团结合，在传感通道中引起符号信号响应（四发射三激发）。即使在较低水平（10 ² cfu mL ⁻¹)，对耐药菌、灭活菌、混合菌的识别准确率高（100%）。此外，开发的传感器阵列在实际样品（猪肉和牛奶）中展示了高通量细菌识别（92.1% 准确率），具有高灵敏度、快速响应、易于操作和特定识别元素的独立性，这将照亮荧光传感器阵列的研制。