Using wastepaper as external carbon sources is an optional way to achieve total nitrogen removal faced with low carbon to nitrogen ratio municipal sewage. Most of studies have primarily focused on using cellulose-rich wastes establishing the separate denitrification units to achieve in-situ fermentation, which can cause blockages and prolong the process chain. In response, a novel in-situ fermentation wastepaper-flora slow-release carbon source (IF-WF) was proposed using in the original denitrification unit. IF-WF could be efficiently utilized in situ and the denitrification rate increased with the increase of nitrate nitrogen. The fermentation products were highly available, but internal acidification of IF-WF inhibited fermentation. Moreover, IF-WF limited the growth of polysaccharides in the extracellular polymeric substances of denitrified sludge. IF-WF finally formed the structure dominated by nitrate-reduction bacteria outside and cellulose-degrading bacteria inside. These results provide guidance for understanding the mechanism of IF-WF for in-situ fermentation to promote nitrogen removal.

面对低碳氮比的城市污水，利用废纸作为外部碳源是实现全氮去除的一种可选方式。大多数研究主要集中在使用富含纤维素的废物建立单独的反硝化单元来实现原位发酵，这可能会导致堵塞并延长工艺链。为此，提出了一种新型原位发酵废纸菌群缓释碳源（IF-WF）用于原反硝化装置。IF-WF可以原位有效利用，反硝化速率随着硝态氮的增加而增加。发酵产物利用率高，但IF-WF内部酸化抑制发酵。而且，IF-WF 限制了反硝化污泥胞外聚合物中多糖的生长。IF-WF最终形成了外硝酸盐还原菌为主，内为纤维素降解菌为主的结构。这些结果为理解 IF-WF 原位发酵促进脱氮的机制提供了指导。