Acta Biomaterialia ( IF 9.4 ) Pub Date : 2023-04-21 , DOI: 10.1016/j.actbio.2023.04.024 Chang Wang 1 , Fang Wang 1 , Wentao Zou 1 , Yawei Miao 1 , Yaowei Zhu 1 , Mengyu Cao 1 , Bing Yu 2 , Hailin Cong 3 , Youqing Shen 4
|
Here, a D-A-D type fluorescent conjugated molecule with a high molar absorption coefficient and emission at 1120 nm in the near-infrared region was synthesized. Conjugated molecules and two polyethylene glycol polymers with different lipophilic ends are assembled into water-soluble nanoparticles to improve their biocompatibility. Then, their physical and chemical properties were studied and compared. Compared with phospholipid-based PEG, styrene-based PEG can reduce the π-π stacking between molecules and the quenching caused by molecular aggregation. It has more advantages in particle size and fluorescence performance and can be better used in biological imaging. In addition, the Nano-particles have good photo-thermal conversion efficiency; the temperature rises to 62.8°C after 980 nm irradiation for 6 min, which can be used as a potential near-infrared II photothermal therapeutic agent. In vivo imaging experiments confirmed that nanomaterials have fluorescence, photoacoustic dual-modal imaging and good biological safety.
Statement of Significance
In this work, we constructed D-A-D type dual donor fluorescent molecules using BBTD, CPDT and EDOT, and used amphiphilic polymers to improve their biocompatibility. Compared with DSPE NPs, PS-NPs can reduce intermolecular π-π stacking and increase quantum yield (QY = 0.98 %). Deep penetration and low biological toxicity make it have biomedical value and realize the integration of multi-functional collaborative imaging. This work can still be further improved and supplemented, and the molecular structure can be optimized to improve its application in biomedical imaging.
中文翻译：
用于荧光/光声成像和综合光热治疗的供体-受体-供体小分子
在这里，合成了在近红外区域具有高摩尔吸收系数和 1120 nm 发射的 DAD 型荧光共轭分子。共轭分子和两个聚乙二醇具有不同亲脂性末端的聚合物被组装成水溶性纳米粒子以提高它们的生物相容性。然后，对它们的物理和化学性质进行了研究和比较。与基于磷脂的PEG相比，基于苯乙烯的PEG可以减少分子之间的π-π堆积和分子聚集引起的猝灭。它在粒径和荧光性能方面更具优势，可以更好地用于生物成像。此外，纳米粒子具有良好的光热转换效率；980 nm照射6 min后温度升至62.8℃，可作为一种潜在的近红外II型光热治疗剂。体内成像实验证实纳米材料具有荧光、光声双模态成像和良好的生物安全性。
重要性声明
在这项工作中，我们使用 BBTD、CPDT 和 EDOT 构建了 DAD 型双供体荧光分子，并使用两亲聚合物来提高它们的生物相容性。与 DSPE NPs 相比，PS-NPs 可以减少分子间 π-π 堆积并提高量子产率 (QY = 0.98 %)。深度渗透和低生物毒性使其具有生物医学价值，实现多功能协同成像一体化。该工作仍可进一步完善和补充，优化分子结构，提高其在生物医学成像方面的应用。
京公网安备 11010802027423号