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Cellular Response and Molecular Mechanism of Glyphosate Degradation by Chryseobacterium sp. Y16C
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-04-21 , DOI: 10.1021/acs.jafc.2c07301 Wenping Zhang 1, 2, 3 , Wen-Juan Chen 1, 2 , Shao-Fang Chen 1, 2 , Qiqi Lei 1, 2 , Jiayi Li 1, 2 , Pankaj Bhatt 4 , Sandhya Mishra 5 , Shaohua Chen 1, 2
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-04-21 , DOI: 10.1021/acs.jafc.2c07301 Wenping Zhang 1, 2, 3 , Wen-Juan Chen 1, 2 , Shao-Fang Chen 1, 2 , Qiqi Lei 1, 2 , Jiayi Li 1, 2 , Pankaj Bhatt 4 , Sandhya Mishra 5 , Shaohua Chen 1, 2
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Glyphosate is one of the most widely used herbicides worldwide. Unfortunately, the continuous use of glyphosate has resulted in serious environmental contamination and raised public concern about its impact on human health. In our previous study, Chryseobacterium sp. Y16C was isolated and characterized as an efficient degrader that can completely degrade glyphosate. However, the biochemical and molecular mechanisms underlying its glyphosate biodegradation ability remain unclear. In this study, the physiological response of Y16C to glyphosate stimulation was characterized at the cellular level. The results indicated that, in the process of glyphosate degradation, Y16C induced a series of physiological responses in the membrane potential, reactive oxygen species levels, and apoptosis. The antioxidant system of Y16C was activated to alleviate the oxidative damage caused by glyphosate. Furthermore, a novel gene, goW, was expressed in response to glyphosate. The gene product, GOW, is an enzyme that catalyzes glyphosate degradation, with putative structural similarities to glycine oxidase. GOW encodes 508 amino acids, with an isoelectric point of 5.33 and a molecular weight of 57.2 kDa, which indicates that it is a glycine oxidase. GOW displays maximum enzyme activity at 30 °C and pH 7.0. Additionally, most of the metal ions exhibited little influence on the enzyme activity except for Cu2+. Finally, with glyphosate as the substrate, the catalytic efficiency of GOW was higher than that of glycine, although opposite results were observed for the affinity. Taken together, the current study provides new insights to deeply understand and reveal the mechanisms of glyphosate degradation in bacteria.
中文翻译：
金黄杆菌降解草甘膦的细胞反应和分子机制。 Y16C
草甘膦是全世界使用最广泛的除草剂之一。不幸的是，草甘膦的持续使用导致了严重的环境污染，并引起了公众对其对人类健康影响的担忧。在我们之前的研究中，金黄杆菌sp。 Y16C 被分离并鉴定为一种高效降解剂，可以完全降解草甘膦。然而，其草甘膦生物降解能力的生化和分子机制仍不清楚。在这项研究中，Y16C 对草甘膦刺激的生理反应在细胞水平上进行了表征。结果表明，在草甘膦降解过程中，Y16C诱导了膜电位、活性氧水平和细胞凋亡等一系列生理反应。 Y16C的抗氧化系统被激活，减轻草甘膦造成的氧化损伤。此外，一种新的基因goW是针对草甘膦而表达的。该基因产物 GOW 是一种催化草甘膦降解的酶，推测结构与甘氨酸氧化酶相似。 GOW编码508个氨基酸，等电点为5.33，分子量为57.2 kDa，表明它是一种甘氨酸氧化酶。 GOW 在 30 °C 和 pH 7.0 时显示最大酶活性。此外，除Cu 2+外，大多数金属离子对酶活性影响不大。最后，以草甘膦为底物，GOW的催化效率高于甘氨酸，尽管亲和力观察到相反的结果。总而言之，当前的研究为深入理解和揭示细菌中草甘膦的降解机制提供了新的见解。
更新日期：2023-04-21
中文翻译：
金黄杆菌降解草甘膦的细胞反应和分子机制。 Y16C
草甘膦是全世界使用最广泛的除草剂之一。不幸的是，草甘膦的持续使用导致了严重的环境污染，并引起了公众对其对人类健康影响的担忧。在我们之前的研究中，金黄杆菌sp。 Y16C 被分离并鉴定为一种高效降解剂，可以完全降解草甘膦。然而，其草甘膦生物降解能力的生化和分子机制仍不清楚。在这项研究中，Y16C 对草甘膦刺激的生理反应在细胞水平上进行了表征。结果表明，在草甘膦降解过程中，Y16C诱导了膜电位、活性氧水平和细胞凋亡等一系列生理反应。 Y16C的抗氧化系统被激活，减轻草甘膦造成的氧化损伤。此外，一种新的基因goW是针对草甘膦而表达的。该基因产物 GOW 是一种催化草甘膦降解的酶，推测结构与甘氨酸氧化酶相似。 GOW编码508个氨基酸，等电点为5.33，分子量为57.2 kDa，表明它是一种甘氨酸氧化酶。 GOW 在 30 °C 和 pH 7.0 时显示最大酶活性。此外，除Cu 2+外，大多数金属离子对酶活性影响不大。最后，以草甘膦为底物，GOW的催化效率高于甘氨酸，尽管亲和力观察到相反的结果。总而言之，当前的研究为深入理解和揭示细菌中草甘膦的降解机制提供了新的见解。
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