使用基于电双层门控场效应晶体管的 DNA 传感器快速检测血清中的大肠杆菌克隆 DNA,Analytical Chemistry

当前位置： X-MOL 学术 › Anal. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

使用基于电双层门控场效应晶体管的 DNA 传感器快速检测血清中的大肠杆菌克隆 DNA
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-04-20 , DOI: 10.1021/acs.analchem.2c05719
Akhil K. Paulose, Yueh-Ju Hou, Yu-Shan Huang, Navyamol Chakkalaparambil Dileep, Chia-Lin Chiu, Arnab Pal, Vishal Mani Kalaimani, Zong-Hong Lin, Chuang-Rung Chang, Cheng-Pin Chen, Yi-Chun Lin, Chien-Yu Cheng, Shu-Hsing Cheng, Chao-Min Cheng, Yu-Lin Wang

在本研究中，开发了一个用于检测大肠杆菌O157:H7的快速诊断平台。基于双电层 (EDL) 门控场效应晶体管的生物传感器 (BioFET) 作为即时检测设备，展示了其高灵敏度、便携性、高选择性、快速响应和易用性。将特别设计的单链DNA探针固定在延伸的栅电极上，结合大肠杆菌的目标互补DNA片段，导致漏极电流在几分钟内急剧变化。目标 DNA 的检测限在缓冲溶液和血清中的浓度为 1 fM。同时，开尔文探针力显微镜的结果表明，在cDNA检测前后，DNA固定化传感器的表面电位降低，这与BioFET漏极电流的降低是一致的。对在质粒中合成的1.2 kb大肠杆菌双链 DNA 进行超声处理，并使用传感器阵列在血清样品中进行检测。凝胶电泳用于通过阐明 DNA 的长度来确认超声处理的效率。这些结果表明，EDL 门控 BioFET 系统是一个很有前途的平台，可以快速识别病原体以满足未来的临床需求。

"点击查看英文标题和摘要"

Rapid Escherichia coli Cloned DNA Detection in Serum Using an Electrical Double Layer-Gated Field-Effect Transistor-Based DNA Sensor

In this study, a rapid diagnosis platform was developed for the detection of Escherichia coli O157:H7. An electrical double layer (EDL)-gated field-effect transistor-based biosensor (BioFET) as a point-of-care testing device is demonstrated with its high sensitivity, portability, high selectivity, quick response, and ease of use. The specially designed ssDNA probe was immobilized on the extended gate electrode to bind the target complementary DNA segment of E. coli, resulting in a sharp drain current change within minutes. The limit of detection for target DNA is validated to a concentration of 1 fM in buffer solution and serum. Meanwhile, the results of a Kelvin probe force microscope were shown to have reduced surface potential of the DNA immobilized sensors before and after the cDNA detection, which is consistent with the decreased drain current of the BioFET. A 1.2 kb E. coli duplex DNA synthesized in plasmid was sonicated and detected in serum samples with the sensor array. Gel electrophoresis was used to confirm the efficiency of sonication by elucidating the length of DNA. Those results show that the EDL-gated BioFET system is a promising platform for rapid identification of pathogens for future clinical needs.

更新日期：2023-04-20

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南