Tenacissoside G alleviated osteoarthritis through the NF-κB pathway both in vitro and in vivo,Immunology Letters

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Tenacissoside G alleviated osteoarthritis through the NF-κB pathway both in vitro and in vivo
Immunology Letters ( IF 3.3 ) Pub Date : 2023-04-20 , DOI: 10.1016/j.imlet.2023.04.007
Xu Cui ₁ , Mengfei Wang ₁ , Hui Li ₁ , Xing Yuwen ₂ , Xiaochan He ₂ , Yangquan Hao ₂ , Chao Lu ₂

Affiliation

Background

Osteoarthritis (OA) is a degenerative joint disease characterized by the destruction of articular cartilage. Tenacissoside G is a flavonoid isolated from the dry roots of Marsdenia tenacissima (Roxb) and has been shown to have anti-inflammatory effects. However, there is no report on the protective effects of Tenacissoside G on OA.

Objectives

To identify the effects and mechanism of Tenacissoside G on OA.

Methods

In vitro, primary mouse chondrocytes were induced with IL-1β to establish OA model. mRNA expression of MMP-13, MMP-3, TNF-α, IL-6 and iNOS, was detected by PCR. Protein expression of Collagen-II, MMP-13, p65, p-p65, and IκBα was detected by Western blot. Collagen-II in chondrocytes was also detected by immunofluorescence. In vivo, we established DMM OA mice model. The preventive effect of Tenacissoside G on OA was observed by micro-CT and histological analysis.

Results

In vitro, Tenacissoside G significantly inhibited the expression of iNOS, TNF-α, IL-6, MMP-3, MMP-13 and the degradation of collagen-II, Tenacissoside G also significantly suppressed NF-κB activation in chondrocytes by IL-1β-stimulated. In vivo, we demonstrated Tenacissoside G can decrease articular cartilage damage and reduce OARSI score.

Conclusion

These results suggest that Tenacissoside G may serve as a potential drug for the prevention and treatment of OA.

中文翻译：

Tenacissoside G 通过 NF-κB 途径在体外和体内减轻骨关节炎

背景

骨关节炎（OA）是一种以关节软骨破坏为特征的退行性关节病。Tenacissoside G 是一种从 Marsdenia tenacissima (Roxb) 的干燥根中分离出来的类黄酮，已被证明具有抗炎作用。但是，目前还没有关于Tenacissoside G对OA的保护作用的报道。

目标

确定 Tenacissoside G 对 OA 的作用和机制。

方法

在体外，用IL-1β诱导原代小鼠软骨细胞建立OA模型。PCR检测MMP-13、MMP-3、TNF-α、IL-6和iNOS的mRNA表达。Western blot 检测 Collagen-II、MMP-13、p65、p-p65 和 IκBα 的蛋白表达。还通过免疫荧光检测了软骨细胞中的胶原蛋白-II。在体内，我们建立了DMM OA小鼠模型。通过显微 CT 和组织学分析观察了 Tenacissoside G 对 OA 的预防作用。

结果

在体外，Tenacissoside G显着抑制iNOS、TNF-α、IL-6、MMP-3、MMP-13的表达和胶原蛋白-II的降解，Tenacissoside G还显着抑制IL-1β在软骨细胞中激活NF-κB -刺激。在体内，我们证明了 Tenacissoside G 可以减少关节软骨损伤并降低 OARSI 评分。