Microchimica Acta ( IF 5.3 ) Pub Date : 2023-04-20 , DOI: 10.1007/s00604-023-05769-0
Pan Xie 1 , Ding Wang 2 , Hongwen Zhao 1 , Na Yin 1 , Shuang Hu 1 , Wenhan Qin 1 , Li Meng 1 , Xin Pan 1 , Yali Yuan 2 , Ruo Yuan 2 , Kanfu Peng 1
|
Fe–N-doped carbon nanomaterials (Fe–N/CMs) were designed as a novel biomimetic enzyme with excellent peroxidase-like activity to achieve high-efficient enzyme cascade catalytic amplification with the aid of glucose oxidase (GOx), which was further combined with target-induced DNA walker amplification to develop a sensitive electrochemical biosensor for thrombin detection. Impressively, massive output DNA was transformed from small amounts of target thrombin by highly effective DNA walker amplification as protein-converting strategy, which could then induce the immobilization of functionalized nanozyme on the electrode surface to achieve the high-efficient electrochemical biomimetic enzyme cascade amplification. As a result, an amplified enzyme cascade catalytic signal was measured for thrombin detection ranging from 0.01 pM to 1 nM with a low detection limit of 3 fM. Importantly, the new biomimetic enzyme cascade reaction coupled the advantages of natural enzyme and nanozyme, which paved an avenue to construct varied artificial multienzymes amplification systems for biosensing, bioanalysis, and disease diagnosis applications.
Graphical Abstract
中文翻译：
电化学仿生酶级联扩增结合靶向诱导DNA步行子检测凝血酶
Fe-N 掺杂碳纳米材料 (Fe-N/CMs) 被设计为一种新型仿生酶，具有优异的类过氧化物酶活性，借助葡萄糖氧化酶 (GOx) 实现高效酶级联催化放大，进一步结合结合靶标诱导的 DNA 步行者扩增，以开发用于凝血酶检测的灵敏电化学生物传感器。令人印象深刻的是，通过高效的 DNA 步行者扩增作为蛋白质转化策略，从少量目标凝血酶转化为大量输出的 DNA，然后可以诱导功能化纳米酶在电极表面的固定化，从而实现高效的电化学仿生酶级联放大。结果，测量了放大的酶级联催化信号，用于凝血酶检测，范围从 0. 01 pM 至 1 nM，检测下限为 3 fM。重要的是，新的仿生酶级联反应结合了天然酶和纳米酶的优势，为构建用于生物传感、生物分析和疾病诊断应用的各种人工多酶放大系统铺平了道路。
京公网安备 11010802027423号