Biochemical Systematics and Ecology ( IF 1.4 ) Pub Date : 2023-04-18 , DOI: 10.1016/j.bse.2023.104641 Dalila da Costa Gonçalves , Wilian Rodrigues Ribeiro , Débora Cristina Gonçalves , Vanessa Sessa Dian , André da Silva Xavier , Águida Aparecida de Oliveira , Luciano Menini , Hélcio Costa
Banana anthracnose is one of the main postharvest diseases economically compromising the fruit production chain. Fruit treatment with natural products can reduce postharvest pathogen infestation and increase protection during storage. For natural control of this disease, the activity of the essential oil of Melaleuca alternifolia (MAEO) was investigated in Colletotrichum musae. The aim of this study was to evaluate the in vitro antifungal activity of the MAEO on C. musae by of different concentrations and to evaluate the effect of MAEO on the incidence and severity of anthracnose. The essential oil (EO) was characterized by gas chromatography (GC-FID and GC-MS). The relative percentage of monoterpenes was 96.94%, and terpinen-4-ol was the main component (47.08%). The minimum inhibitory dose (EC100) of the mycelial growth of C. musae was reached at a concentration of 2.15 μg mL−1. Bananas were treated with water emulsions MAEO into at different concentrations (0.50, 1.00, 2.00, 3.00, 4.00 and 5.00 μg mL−1) for 08 days of storage at 25 ± 2 °C. MAEO showed fungistatic action from the concentration of 2.0 μg mL−1 (p < 0.05). The results showed that, after storage, MAEO at 0.50, 1.00, 2.00, 3.00, 4.00 and 5.00 μg mL−1 reduced area under the disease-progress curve (AUDPC) by 80%, 90%, 95%, 96%, 97% and 98%, respectively. MAEO reduces the severity of anthracnose after fruits treatment, without causing phytotoxic action, maybe a promising candidate for Biorational pesticides, in preservation and increment shelf life fruits banana.
中文翻译:
使用互叶白千层精油作为延长香蕉果实保质期的有效策略
香蕉炭疽病是影响水果生产链经济的主要采后病害之一。用天然产品处理水果可以减少采后病原体侵染并增加贮藏期间的保护。为了自然控制这种疾病,研究了互叶白千层(MAEO)精油在炭疽病菌中的活性。本研究的目的是评估不同浓度的 MAEO 对C. musae的体外抗真菌活性,并评估 MAEO 对炭疽病发病率和严重程度的影响。精油(EO)通过气相色谱法表征(GC-FID 和 GC-MS)。单萜类相对百分含量为96.94%,主要成分为萜品烯-4-醇(47.08%)。C. musae菌丝体生长的最小抑制剂量 (EC100)在浓度为 2.15 μg mL -1时达到。将香蕉用水乳剂 MAEO 处理成不同浓度(0.50、1.00、2.00、3.00、4.00 和 5.00 μg·mL-1),在 25 ± 2 °C 下储存 8 天。MAEO 在 2.0 μg mL -1的浓度下显示出抑制真菌的作用(p < 0.05)。结果表明,储存后,MAEO 的浓度为 0.50、1.00、2.00、3.00、4.00 和 5.00 μg·mL-1疾病进展曲线下面积 (AUDPC) 分别减少了 80%、90%、95%、96%、97% 和 98%。MAEO 在水果处理后降低炭疽病的严重程度,而不会引起植物毒性作用,可能是 Biorational 杀虫剂的有前途的候选者,用于保鲜和延长水果香蕉的保质期。