人参总皂苷及人参提取物对环磷酰胺所致小鼠免疫抑制的预防作用,Phytotherapy Research

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人参总皂苷及人参提取物对环磷酰胺所致小鼠免疫抑制的预防作用
Phytotherapy Research ( IF 6.1 ) Pub Date : 2023-04-18 , DOI: 10.1002/ptr.7836
He Zhang _{1,

2} , Yue Sun ₃ , Meiling Fan ₁ , Yuyao Zhang ₂ , Zuguo Liang ₄ , Lancao Zhang ₂ , Xiang Gao ₄ , Xinzhu He ₄ , Xiangyan Li ₂ , Daqing Zhao ₂ , Gianni Sagratini ₃ , Hang Su ₂ , Wenxiu Qi ₂

Affiliation

口服汤剂在中药中应用广泛。汤剂中的多糖促进小分子的暴露并增加其生物利用度。本研究主要比较了人参总皂苷（TGS）和人参提取物（GE）对环磷酰胺诱导的免疫抑制小鼠的成分和活性。32只小鼠随机分为对照组、模型组、TGS组和GE组。小鼠口服给药28天，然后在最后四天注射环磷酰胺。成分分析结果显示，TGS中12种人参皂苷的总含量（67.21%）高于GE（2.04%）；TGS中17种氨基酸的总含量（1.41%）低于GE（5.36%）；TGS（74.12%）和GE（76.36%）中10种单糖的总含量相似。动物实验结果表明，TGS和GE均通过抑制细胞凋亡保护骨髓造血功能，恢复骨髓正常细胞周期；维持Th1和Th2细胞之间的动态平衡；还保护脾脏、胸腺和肝脏。同时，TGS和GE通过增加乳酸菌的丰度并减少臭杆菌和梭菌_UCG-014的丰度来保护免疫抑制小鼠的肠道细菌。GE的预防效果在某些参数上优于TGS。总之，TGS和GE可以保护环磷酰胺诱导的免疫抑制小鼠的免疫功能。同时，与TGS相比，GE表现出更高的生物利用度和生物活性，因为多糖和人参皂苷的协同作用在保护免疫功能方面发挥着重要作用。

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Prevention effect of total ginsenosides and ginseng extract from Panax ginseng on cyclophosphamide-induced immunosuppression in mice

Oral decoction is widely applied in traditional Chinese medicines. The polysaccharides of decoction promote the exposure of small molecules and increase their bioavailability. This study mainly compared the component and activities of total ginsenosides (TGS) and ginseng extract (GE) on immunosuppressed mice induced by cyclophosphamide. Thirty-two mice were randomly divided into control, model, TGS, and GE groups. The mice were orally administered for 28 days and then injected with cyclophosphamide on the last four days. The results of component analysis showed the total content of 12 ginsenosides in TGS (67.21%) was higher than GE (2.04%); the total content of 17 amino acids in TGS (1.41%) was lower than GE (5.36%); the total content of 10 monosaccharides was similar in TGS (74.12%) and GE (76.36%). The animal results showed that both TGS and GE protected the hematopoietic function of bone marrow by inhibiting cell apoptosis, and recovering the normal cell cycle of BM; maintained the dynamic balance between the Th1 and Th2 cells; also protected the spleen, thymus, and liver. Meanwhile, TGS and GE protected the intestinal bacteria of immunosuppressed mice by increasing the abundance of lactobacillus and decreasing the abundance of the odoribacter and clostridia_UCG-014. The prevention effect of GE was superior to TGS in some parameters. In conclusion, TGS and GE protected the immune function of immunosuppressed mice induced by cyclophosphamide. Meanwhile, GE showed higher bioavailability and bioactivity compared with TGS, because the synergistic effect of polysaccharides and ginsenosides plays an important role in protecting the immune function.

更新日期：2023-04-18

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