Currently, most nanofiltration membranes exhibit satisfactory separation performance on the anions or cations, and few membranes could concurrently separate anion and cation. Therefore, preparing nanofiltration membranes with excellent separation performance for both anionic and cationic ions remains a challenge. Herein, we have developed a series of nanofiltration membranes using conventional interfacial polymerization with different ratios of trimesoyl chloride (TMC) and piperazine (PIP) concentrations. In the interface polymerization process, the amine groups carried by PIP imparted a positive charge, while the hydrolysis of acyl chloride groups resulted in the generation of carboxylic acid groups that introduced a negative charge. The mix-charged nanofiltration membrane could be fabricated by varying PIP/TMC concentration ratios. The optimized membrane exhibited high rejection rates for bivalent ions (Na₂SO₄ rejection of 96.6 %, MgCl₂ rejection of 93.0 %), and its selectivity for Mg²⁺/Li⁺ mixed solution was improved significantly. This work provides a novel approach for the development of nanofiltration membranes capable of effectively separating mono-/divalent ions.