基于沉淀的丝素蛋白快速胶凝、高粘附性和磁性纳米复合水凝胶用于修复不规则骨缺损,Advanced Functional Materials

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基于沉淀的丝素蛋白快速胶凝、高粘附性和磁性纳米复合水凝胶用于修复不规则骨缺损
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2023-04-16 , DOI: 10.1002/adfm.202302442
Yan‐Pei Zou ₁ , Hai‐Feng Liang ₁ , Ben Wang ₁ , Qi‐Chen Zhang ₁ , Di‐Han Su ₁ , Shun‐Yi Lu ₁ , Qian‐Yi Zhang ₁ , Tao Wu ₁ , Lan Xiao _{2,

3} , Yin Xiao _{2,

3,

4} , Jian Dong ₁ , Li‐Bo Jiang ₁ , Xi‐Lei Li ₁

Affiliation

临界尺寸的骨缺损，尤其是不规则形状的骨缺损，仍然是骨科领域的重大挑战。尽管开发了各种用于骨再生的生物材料，但它们在修复不规则骨缺损方面的应用受到复杂的制备过程的限制，并且缺乏形状适应能力、生理粘附性和有效的成骨生物活性。在本研究中，通过引入具有丰富酚羟基的单宁酸（TA）和Fe ₃ O ₄纳米颗粒作为金属酚网络（MPN），开发了一种简单的沉淀策略，可以轻松制备快速胶凝、形状适应性的材料。、高粘合性再生丝素蛋白(RSF)/TA/Fe ₃ O ₄可以响应静磁场（SMF）的水凝胶系统。RSF/TA/Fe ₃ O ₄水凝胶在生物微环境中表现出足够的粘附力，并且在外部SMF下在体外和体内表现出良好的成骨效果，因此可用于修复临界尺寸的骨缺损。_{此外，生物信息学分析揭示了Fe 3} O ₄的协同机制NPs和SMF的成骨作用可以通过激活环磷酸鸟苷（cGMP）/蛋白激酶G（PKG）/细胞外信号调节激酶（ERK）信号通路来促进成骨细胞分化。这项研究提供了一种有前途的生物材料，具有潜在的临床应用价值，可用于未来治疗（不规则）临界尺寸的骨缺损。

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Precipitation-Based Silk Fibroin Fast Gelling, Highly Adhesive, and Magnetic Nanocomposite Hydrogel for Repair of Irregular Bone Defects

Critical-sized bone defects, especially for irregular shapes, remain a significant challenge in orthopedics. Although various biomaterials are developed for bone regeneration, their application for repair of irregular bone defects is limited by the complicated preparation procedures involved, and their lack of shape-adaptive capacity, physiological adhesion, and potent osteogenic bioactivity. In the present study, a simple strategy of precipitation by introducing tannic acid (TA) with abundant phenolic hydroxyl groups and Fe₃O₄ nanoparticles, as metal-phenolic networks (MPN), is developed to easily prepare a fast gelling, shape-adaptive, and highly adhesive regenerated silk fibroin (RSF)/TA/Fe₃O₄ hydrogel system that can respond to a static magnetic field (SMF). The RSF/TA/Fe₃O₄ hydrogel exhibits sufficient adhesion in biological microenvironments and good osteogenic effect in vitro and in vivo, under an external SMF, and thus, can be applied to repair critical-sized bone defects. Moreover, bioinformatics analysis reveals that the synergistic mechanism of Fe₃O₄ NPs and SMF on osteogenic effects can be promotion of osteoblast differentiation via activation of the cyclic guanosine monophosphate (cGMP)/protein kinase G (PKG)/extracellular signal-regulated kinase (ERK) signaling pathway. This study provides a promising biomaterial with potential clinical application for the future treatment of (irregular) critical-sized bone defects.

更新日期：2023-04-16

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