在需要生物合成替代生物医学药物以预防和治疗感染的推动下，氧化铜纳米粒子 (CuONPs) 已成为一种有前途的途径。源自蓝藻的 CuONPs 合成具有重要意义，因为它提供了一种生态友好、具有成本效益且具有生物相容性的途径。在本研究中，对生物合成的 CuONPs 进行了表征，并研究了它们的毒性。使用 TEM、SEM 和 AFM 的形态分析显示球形粒径为 20.7 nm，铜含量为 96%，这证实了 CuONPs 的纯度。IC 50值为 64.6 µg ml -1的生物源 CuONP在超氧自由基清除试验中显示 90% 的自由基清除率。CuONPs 通过 86% 的蛋白质变性显示出增强的抗炎活性，IC 为50值为 89.9 µg ml -1。生物源性 CuONPs 对细菌菌株表现出显着的毒性，蜡样芽孢杆菌的最低 MIC 值为 62.5 µg ml -1 ，而白色念珠菌的MIC 值为 125 µg ml -1。此外，CuONPs 在与标准药物结合时表现出高度的协同相互作用。CuONPs 对非小细胞肺癌表现出显着的细胞毒性，A549 的IC 50值为 100.8 µg ml -1和 88.3 µg ml -1对于具有凋亡活性的 H1299 细胞系。此外，还评估了生物源性 CuONPs 对亚甲蓝染料的光催化降解潜力，并能够在 90 分钟内去除 94% 的染料。自由基清除分析表明，CuONPs 辅助染料降解主要是由氢氧根引起的。Biogenic CuONPs 似乎是一种环保且具有成本效益的光催化剂，用于处理被合成染料污染的废水，这些染料对水生生物群和人类健康构成威胁。本研究强调了Phormidium衍生的 CuONPs 的生物医学和光催化潜力的融合，作为未来在纳米医学和生物修复中应用的有吸引力的方法。
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Toxicological assessment of Phormidium sp. derived copper oxide nanoparticles for its biomedical and environmental applications
Driven by the need to biosynthesized alternate biomedical agents to prevent and treat infection, copper oxide nanoparticles (CuONPs) have surfaced as a promising avenue. Cyanobacteria-derived synthesis of CuONPs is of substantive interest as it offers an eco-friendly, cost-effective, and biocompatible route. In the present study biosynthesized CuONPs were characterized and investigated regarding their toxicity. Morphological analysis using TEM, SEM and AFM showed the spherical particle size of 20.7 nm with 96% copper that confirmed the purity of CuONPs. Biogenic CuONPs with IC50 value of 64.6 µg ml−1 showed 90% scavenging of free radicals in superoxide radical scavenging assay. CuONPs showed enhanced anti-inflammatory activity by 86% of protein denaturation with IC50 value of 89.9 µg ml−1. Biogenic CuONPs exhibited significant toxicity against bacterial strains with lowest MIC value of 62.5 µg ml−1 for B. cereus and fungal strain with a MIC value of 125 µg ml−1 for C. albicans. In addition CuONPs demonstrated a high degree of synergistic interaction when combined with standard drugs. CuONPs exhibited significant cytotoxicity against non-small cell lung cancer with an IC50 value of 100.8 µg ml−1 for A549 and 88.3 µg ml−1 for the H1299 cell line with apoptotic activities. Furthermore, biogenic CuONPs was evaluated for their photocatalytic degradation potential against methylene blue dye and were able to removed 94% dye in 90 min. Free radical scavenging analysis suggested that CuONPs assisted dye degradation was mainly induced by hydroxide radicals. Biogenic CuONPs appears as an eco-friendly and cost effective photocatalyst for the treatment of wastewater contaminated with synthetic dyes that poses threat to aquatic biota and human health. The present study highlighted the blend of biomedical and photocatalytic potential of Phormidium derived CuONPs as an attractive approach for future applications in nanomedicine and bioremediation.
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