Bacterial cellulose (BC) has good network structure, biocompatibility, and excellent mechanical properties, and is widely used in the field of biomaterials. The controllable degradation of BC can further broaden its application. Oxidative modification and cellulases may endow BC with degradability, but these methods inevitably lead to the obvious reduction of its initial mechanical properties and uncontrolled degradation. In this paper, the controllable degradation of BC was realized for the first time by using a new controlled release structure that combines the immobilization and release of cellulase. The immobilized enzyme has higher stability and is gradually released in the simulated physiological environment, and its load can control the hydrolysis rate of BC well. Furthermore, the BC-based membrane prepared by this method retains the favorable physicochemical performance of the original BC, including flexibility and great biocompatibility, and holds good application prospects in drug control release or tissue repair.