Natural polymer derived hydrogel bioink with enhanced thixotropy improves printability and cellular preservation in 3D bioprinting,Journal of Materials Chemistry B

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Natural polymer derived hydrogel bioink with enhanced thixotropy improves printability and cellular preservation in 3D bioprinting
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2023-04-14 , DOI: 10.1039/d2tb02786k
Rongwei Cui ₁ , Sumei Li ₁ , Taiyi Li ₁ , Xue Gou ₂ , Tao Jing ₂ , Guowei Zhang _{1,

2} , Guihua Wei ₁ , Zhongmin Jin ₃ , Xiong Xiong ₁ , Shuxin Qu ₂

Affiliation

Three-dimensional (3D) bioprinting is evolving into a promising technology by spatially controlling the distribution of living cells for the biomedical field. However, maintaining high printability while protecting cells from damage due to shear stress remains the key challenge for extrusion-based 3D bioprinting. Herein, we developed a novel “protein–polyphenol–polysaccharide” extrusion-based bioink named Gel-TA-Alg@Ca²⁺ using gelatin (Gel), tannic acid (TA) and sodium alginate (Alg) with quantitative thixotropy by pre-crosslinking with a series of low concentrations of CaCl₂ at 0.03, 0.04, 0.05 and 0.06 M, respectively. Our experimental design quantitatively presented the positive proportional functional relationship between the thixotropy of Gel-TA-Alg@Ca²⁺ and printability (including injectability and formability) for the first time. Importantly, the thixotropy proportionately and significantly elevated cellular viability after 3D bioprinting due to the reduced extrusion force involved in printing. 3D bioprinted constructs composed of Gel-TA-Alg@Ca²⁺ and MG-63 cells exhibited a good cell viability rate for more than 14 days. These findings provide valuable insights into the rational design of thixotropic bioink and offer more opportunities to probe the relationship between the thixotropy and the success of 3D bioprinting.

中文翻译：

具有增强触变性的天然聚合物衍生水凝胶生物墨水可改善 3D 生物打印中的可打印性和细胞保存

三维 (3D) 生物打印通过在空间上控制生物医学领域的活细胞分布，正在发展成为一项有前途的技术。然而，在保持高印刷适性的同时保护细胞免受剪切应力造成的损害仍然是基于挤压的 3D 生物打印的主要挑战。在此，我们使用明胶（Gel）、单宁酸（TA）和海藻酸钠（Alg）开发了一种新型的基于“蛋白质-多酚-多糖”挤出的生物墨水，名为 Gel-TA-Alg@Ca ²⁺与一系列分别为 0.03、0.04、0.05 和 0.06 M的低浓度 CaCl _2交联。^{我们的实验设计定量呈现了Gel-TA-Alg@Ca 2+}的触变性与触变性之间的正比例函数关系和印刷适性（包括可注射性和成型性）首次。重要的是，由于打印中涉及的挤压力降低，触变性在 3D 生物打印后成比例且显着提高了细胞活力。^{由 Gel-TA-Alg@Ca 2+}和 MG-63 细胞组成的 3D 生物打印结构在超过 14 天的时间里表现出良好的细胞存活率。这些发现为触变性生物墨水的合理设计提供了宝贵的见解，并为探索触变性和 3D 生物打印成功之间的关系提供了更多机会。

更新日期：2023-04-14

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