家蚕肽聚糖识别蛋白S5促进家蚕Cypovirus 1的增殖,Journal of Agricultural and Food Chemistry

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家蚕肽聚糖识别蛋白S5促进家蚕Cypovirus 1的增殖
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-04-13 , DOI: 10.1021/acs.jafc.3c00927
Feifei Ren ₁ , Jiming Yan ₁ , Xiong Wang ₁ , Yukai Xie ₁ , Nan Guo ₁ , Luc Swevers ₂ , Jingchen Sun ₁

Affiliation

Bombyx mori cypovirus 1 (BmCPV1) 是家蚕的主要病原体，是属于呼肠孤病毒科的典型 dsRNA病毒。在本研究中，通过对 BmCPV1 感染后的家蚕中肠进行 RNA-seq 分析，共鉴定出 2520 个差异表达基因（DEGs），基因本体论（GO）功能注释表明，DEGs 主要在结合（分子功能）、细胞（细胞成分）和细胞过程（生物过程）。此外，京都基因和基因组百科全书 (KEGG) 功能注释显示，DEG 主要分布在全局和概览代谢图谱、翻译和信号转导中。在已确定的 DEG 中，BmPGRP-S5属于肽聚糖识别蛋白 (PGRP) 家族。以前的研究表明，PGRPs 参与了家蚕和 BmCPV1 之间的相互作用。在这里，我们探讨了 BmPGRP-S5 对 BmCPV1 复制的影响，并证明 BmPGRP-S5 通过过表达或敲低实验促进 BmCPV1 在 BmN 细胞中的增殖。家蚕幼虫中BmPGRP-S5的敲低同样促进了 BmCPV1 的增殖。通过实验验证，我们因此确定 BmPGRP-S5 作为 BmCPV1 感染的前病毒宿主因子。本研究阐明了 BmCPV1 的增殖机制，并为 BmPGRP-S5 的功能作用提供了新的见解。

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Peptidoglycan Recognition Protein S5 of Bombyx mori Facilitates the Proliferation of Bombyx mori Cypovirus 1

Bombyx mori cypovirus 1 (BmCPV1), a primary pathogen of the silkworm, is a typical dsRNA virus belonging to the Reoviridae family. In this study, a total of 2520 differentially expressed genes (DEGs) were identified by RNA-seq analysis of the silkworm midgut after BmCPV1 infection and Gene Ontology (GO) functional annotation showed that the DEGs predominantly functioned in binding (molecular function), cell (cellular component), and cellular processes (biological process). Additionally, the Kyoto Encyclopedia of Genes and Genomes (KEGG) functional annotation revealed that the DEGs were mainly distributed in global and overview metabolism maps, translation, and signal transduction. Among the identified DEGs, BmPGRP-S5 belongs to the peptidoglycan recognition protein (PGRP) family. Previous studies have revealed that PGRPs were involved in the interactions between silkworm and BmCPV1. Here, we explored the effect of BmPGRP-S5 on BmCPV1 replication and demonstrated that BmPGRP-S5 promotes the proliferation of BmCPV1 in BmN cells through overexpression or knockdown experiments. Knocking down of BmPGRP-S5 in silkworm larvae similarly promoted the proliferation of BmCPV1. Through experimental validation, we therefore determined that BmPGRP-S5 acts as a proviral host factor for BmCPV1 infection. This study clarifies the proliferation mechanism of BmCPV1 and provides new insights into the functional role of BmPGRP-S5.

更新日期：2023-04-13

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