Rapid and Ultrasensitive Fluorescence Sensing Platform Based on Nanometer-Sized Metal–Organic Frameworks for Transgenic CaMV 35S Promoter Detection,ACS Applied Nano Materials

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Rapid and Ultrasensitive Fluorescence Sensing Platform Based on Nanometer-Sized Metal–Organic Frameworks for Transgenic CaMV 35S Promoter Detection
ACS Applied Nano Materials ( IF 5.3 ) Pub Date : 2023-04-12 , DOI: 10.1021/acsanm.3c01091
Yin Liu ₁ , Shiying Zhou ₁ , Jiangbo Dong ₁ , Human Sun ₁ , Liyuan Deng ₁ , Yi Ma ₂ , Dong Zhao ₃ , Danqun Huo _{1,

4} , Changjun Hou _{1,

2}

Affiliation

Rapid and sensitive identification of genetically modified organisms (GMOs) is urgent for food safety and people health. Herein, a rapid and ultrasensitive fluorescence sensing platform is developed based on the single-strand DNA-labeled FAM dye (FAM-ssDNA) and nanometer-sized Fe-metal–organic framework (Fe-MOF), Fe-MIL-88. Fe-MIL-88 possesses a large specific surface area, remarkable preferential combining capacity with ssDNA versus dsDNA, and great quenching ability. FAM-ssDNA probes are elaborately designed as facile fluorescent probes to be fully complementary with the target of cauliflower mosaic virus 35S promoter (CaMV 35S), which can be absorbed to the surface of Fe-MIL-88 through π–π stacking and electrostatic interaction. The results indicate that the quenching ability of Fe-MIL-88 is attributed to the combination of photoinduced electron transfer (PET) and fluorescence resonance energy transfer (FRET) processes. Benefiting from the distinguishable ability of Fe-MIL-88, the fluorescent biosensor exhibits a satisfactory linear range from 5 pM to 50 nM and a low limit of detection (LOD) down to 0.184 pM. Also, the operation time was less than 1 h. Furthermore, the sensor also performs well in the anti-interference test and real sample test. Importantly, the sensing platform is equipped with easier operation without any complicated and difficult immobilization steps. Therefore, the sensor owns cheering potential for the detection of GMOs.

中文翻译：

基于纳米级金属有机骨架的快速超灵敏荧光传感平台用于转基因 CaMV 35S 启动子检测

快速、灵敏地识别转基因生物 (GMO) 对食品安全和人类健康至关重要。在此，基于单链 DNA 标记的 FAM 染料 (FAM-ssDNA) 和纳米级铁金属有机框架 (Fe-MOF) Fe-MIL-88 开发了一种快速超灵敏荧光传感平台。Fe-MIL-88 具有较大的比表面积、与 ssDNA 相比与 dsDNA 显着的优先结合能力以及强大的猝灭能力。FAM-ssDNA 探针被精心设计为易荧光探针，与花椰菜花叶病毒 35S 启动子 (CaMV 35S) 的靶标完全互补，可通过 π-π 堆积和静电相互作用吸附到 Fe-MIL-88 表面. 结果表明，Fe-MIL-88 的猝灭能力归因于光诱导电子转移 (PET) 和荧光共振能量转移 (FRET) 过程的结合。受益于 Fe-MIL-88 的可区分能力，荧光生物传感器表现出从 5 pM 到 50 nM 的令人满意的线性范围和低至 0.184 pM 的检测下限 (LOD)。此外，操作时间少于 1 小时。此外，该传感器在抗干扰测试和实样测试中也表现良好。重要的是，传感平台配备了更简单的操作，无需任何复杂和困难的固定步骤。因此，该传感器具有检测转基因的潜力。受益于 Fe-MIL-88 的可区分能力，荧光生物传感器表现出从 5 pM 到 50 nM 的令人满意的线性范围和低至 0.184 pM 的检测下限 (LOD)。此外，操作时间少于 1 小时。此外，该传感器在抗干扰测试和实样测试中也表现良好。重要的是，传感平台配备了更简单的操作，无需任何复杂和困难的固定步骤。因此，该传感器具有检测转基因的潜力。受益于 Fe-MIL-88 的可区分能力，荧光生物传感器表现出从 5 pM 到 50 nM 的令人满意的线性范围和低至 0.184 pM 的检测下限 (LOD)。此外，操作时间少于 1 小时。此外，该传感器在抗干扰测试和实样测试中也表现良好。重要的是，传感平台配备了更简单的操作，无需任何复杂和困难的固定步骤。因此，该传感器具有检测转基因的潜力。传感平台操作更简单，无需任何复杂困难的固定步骤。因此，该传感器具有检测转基因的潜力。传感平台操作更简单，无需任何复杂困难的固定步骤。因此，该传感器具有检测转基因的潜力。

更新日期：2023-04-12

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