CaP_PILP 交联改善脱矿牙本质性能并协同促进仿生矿化,ACS Omega

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CaP_PILP 交联改善脱矿牙本质性能并协同促进仿生矿化
ACS Omega ( IF 3.7 ) Pub Date : 2023-04-11 , DOI: 10.1021/acsomega.2c07825
Lin Tang ₁ , Lingli Zhu ₁ , Yuhua Liu ₁ , Yi Zhang ₂ , Bowen Li ₃ , Mei Wang ₄

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目的：探讨最佳交联（化学处理）对脱矿牙本质基质的影响以及与磷酸钙聚合物诱导液态前驱体（CaP-PILP）仿生再矿化（物理处理）可能的协同作用，为树脂的临床应用提供有益的参考。牙本质粘合和牙本质过敏。方法：用戊二醛 (GA)、碳二亚胺 (EDC) 和原花青素 (PA) 处理脱矿牙本质进行交联，然后进行 CaP-PILP 仿生再矿化。无论修饰如何，都对形态、表面机械和物理化学性质以及酶抗性进行了评估。结果：胶原纤维形态完整，表面显微硬度较高，GA、PA 和 EDC 交联后可见酰胺 I-III 带的特征峰。在未经处理的脱矿牙本质中观察到胶原蛋白塌陷和溶解，受到酶的攻击，而胶原纤维结构在 GA 和 PA 处理的标本中保持完整。PA 交联和矿化后 2 天时可见层状矿物相，牙本质小管在 4-6 天时几乎完全封闭。然而，在第8天，对照组的牙本质小管之间仍然可见脱矿的胶原纤维和开放的小管。结论：与 EDC 和未处理相比，GA 和 PA 交联可以显着保留胶原纤维网络的结构完整性、酶抗性和机械性能。同时，在 PA 中观察到 CaP-PILP 对仿生再矿化的最强协同作用，并进一步显着提高质量并缩短矿化持续时间。

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Crosslinking Improve Demineralized Dentin Performance and Synergistically Promote Biomimetic Mineralization by CaP_PILP

Objective: to explore the effects of optimal crosslinking (chemical treatment) on demineralized dentin matrix and the possible synergism with calcium phosphate polymer-induced liquid precursor (CaP-PILP) bionic remineralization (physical treatment), and offer benefit to the clinic of resin–dentin bonding and dentin hypersensitivity. Methods: demineralized dentin was treated with glutaraldehyde (GA), carbodiimide (EDC), and procyanidin (PA) for crosslinking, followed by CaP-PILP biomimetic remineralization. The morphology, surface mechanical and physio-chemical properties, and enzymatic resistance were evaluated regardless of the modification. Results: the collagen fibers appeared morphologically complete with higher surface microhardness and characteristic peaks of amide I–III bands were visible after GA, PA, and EDC crosslinking. Collagen collapse and dissolution were seen in untreated demineralized dentin with enzyme attack, while the collagen fiber structure remained intact in GA- and PA-treated specimens. The lamellar mineral phase was visible at 2 days and the dentin tubules were almost completely enclosed at 4–6 days after PA crosslinking and mineralization. However, demineralized collagen fibers and open tubules were still visible between the dentinal tubules on day 8 in the control group. Conclusion: the structure integrity, enzyme resistance, and mechanical properties of the collagen fiber network could be significantly preserved by GA and PA crosslinking than EDC and no treatment. While, strongest synergistic effects were observed in PA on bionic remineralization by CaP-PILP, and further significantly improve the quality and shorten the duration of mineralization. These findings would be beneficial for dental clinical practice of resin–dentin bonding and dentin hypersensitivity.

更新日期：2023-04-11

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