Integrated fixed-film activated sludge (IFAS) system has considerable advantages in treating aniline wastewater economically and efficiently. However, the response mechanism of IFAS to aniline needs further study. Herein, IFAS in continuous-flow (CF-IFAS) and batch mode (B-IFAS) were set up to investigate it. The removal efficiency of aniline exceeded 99% under different stress intensities. At low stress intensity (aniline ≈ 200 mg/L), the total nitrogen removal efficiency of B-IFAS was approximately 37.76% higher than CF-IFAS. When the stress intensity increased (aniline ≥ 400 mg/L), both were over 82%. CF-IFAS was restrained by denitrification while nitrification in B-IFAS. The legacy effect of perturbation of B-IFAS made microflora quickly reach new stability. The closer interspecific relationship in B-IFAS and more key species: Leucobacter, Rhodococcus, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Ellin6067 and norank_f_NS9_marine_group. Metabolic and Cell growth and death were the most abundant metabolic pathways, resulting both systems the excellent pollutant removal and stability under high stress intensity.

集成固定膜活性污泥（IFAS）系统在经济高效地处理苯胺废水方面具有相当大的优势。但IFAS对苯胺的反应机制有待进一步研究。在此，设置了连续流 (CF-IFAS) 和批处理模式 (B-IFAS) 的 IFAS 来对其进行研究。不同应力强度下苯胺去除率均超过99%。在低胁迫强度下（苯胺 ≈ 200 mg/L），B-IFAS 的总氮去除效率比 CF-IFAS 高约 37.76%。当应力强度增加时（苯胺≥400 mg/L），两者均超过82%。CF-IFAS 受到反硝化作用的抑制，而 B-IFAS 中的硝化作用受到抑制。B-IFAS 扰动的遗留效应使微生物群落迅速达到新的稳定性。B-IFAS 中更紧密的种间关系和更多关键物种：白杆菌属、红球菌属、异根瘤菌-新根瘤菌-副根瘤菌-根瘤菌、Ellin6067 和 norank_f_NS9_marine_group。代谢和细胞生长和死亡是最丰富的代谢途径，导致这两个系统在高压力强度下具有出色的污染物去除和稳定性。