The Key Effect of Carboxyl Group and CuN2O2 Coordinate Structure for Cu, N Co-Doped Carbon Dots with Peroxidase-Like Property,Small

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The Key Effect of Carboxyl Group and CuN2O2 Coordinate Structure for Cu, N Co-Doped Carbon Dots with Peroxidase-Like Property
Small ( IF 13.0 ) Pub Date : 2023-04-08 , DOI: 10.1002/smll.202300883
Yurong Ma ₁ , Mengling Zhang _{1,

2} , Jie Wu ₁ , Yajie Zhao ₁ , Xin Du ₁ , Hui Huang ₁ , Yunjie Zhou ₁ , Yang Liu ₁ , Zhenhui Kang _{1,

2}

Affiliation

Carbon dots (CDs) with good water solubility and biocompatibility have become a research hotspot in the nano-enzyme and biomedical field. However, the problems of low catalytic activity and ambiguous catalytic site of CDs as nanozymes still need to be addressed. In this work, CDs loaded with Cu single atoms are obtained through pyrolysis, and the coordination structure and surface functional groups are regulated by adjusting the pyrolysis temperature. CDs obtained at 300 °C (named Cu-CDs-300) have the most carboxyl content and Cu is coordinated in the form of CuN₂O₂, which can better decompose H₂O₂ to produce free radical and is beneficial to catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). The v_max is 6.56*10⁻⁷ m s⁻¹, 6.56 times higher than that of horseradish peroxidase (HRP). Moreover, Cu-CDs-300 can effectively lead to CT26 apoptosis by generating much free radicals. This work demonstrates the synergistic effect of oxygen-containing functional groups and metal coordination structures on peroxide-like activity of CDs and provides new ideas for the design of clear active structure and high efficiency peroxide-like single atom CDs catalyst.

中文翻译：

羧基和CuN2O2配位结构对具有类过氧化物酶性质的Cu、N共掺杂碳点的关键作用

碳点（CDs）具有良好的水溶性和生物相容性，已成为纳米酶和生物医学领域的研究热点。然而，CDs作为纳米酶的催化活性低和催化位点不明确的问题仍然需要解决。本工作通过热解获得负载Cu单原子的碳点，并通过调节热解温度来调节配位结构和表面官能团。_{300℃获得的CD（命名为Cu-CDs-300）羧基含量最高，Cu以CuN 2} O ₂形式配位，能更好地分解H ₂ O ₂产生自由基，有利于催化3,3',5,5'-四甲基联苯胺 (TMB) 的氧化。v _max为6.56*10 ^-7m s ^{-1 ，是辣根过氧化物酶(HRP)}的6.56倍。此外，Cu-CDs-300可以通过产生大量自由基，有效导致CT26细胞凋亡。该工作证明了含氧官能团和金属配位结构对环糊精类过氧化物活性的协同作用，为设计清晰的活性结构和高效类过氧化物单原子环糊精催化剂提供了新思路。

更新日期：2023-04-08

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