白蚁士兵和工蚁触角化学感应基因的基因表达谱,Insect Molecular Biology

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白蚁士兵和工蚁触角化学感应基因的基因表达谱
Insect Molecular Biology ( IF 2.3 ) Pub Date : 2023-04-05 , DOI: 10.1111/imb.12841
Ryohei H Suzuki ₁ , Takumi Hanada ₁ , Yoshinobu Hayashi ₂ , Shuji Shigenobu ₃ , Kiyoto Maekawa ₄ , Masaru K Hojo ₅

Affiliation

白蚁有一个复杂的社会系统，涉及群体成员之间的合作和分工。尽管这个社会系统受到群体中产生的化学信号的调节，但仍不清楚其他成员如何感知这些信号。众所周知，信号转导是由触角中的一些结合蛋白接收气味分子而触发的，然后信号被传输到化学感应受体。然而，关于白蚁信号转导中化学感应基因的作用的信息不足。在这里，我们鉴定了白蚁Reticulitermes speratus中参与化学感应接收的基因并对工人和士兵触角进行了全基因组比较转录组分析。首先，我们从基因组数据中鉴定出 31 种气味结合蛋白 (OBP) 和 3 种化学感应蛋白 A (CheA)。此后，我们进行了 RNA 测序，以比较OBP、CheAs和先前确定的工人和士兵触角之间的化学感应受体基因的表达水平。种姓之间的表达没有显着差异的受体基因。然而，三种非受体气味检测/结合蛋白（OBP、CheA和感觉神经元膜蛋白）的表达水平）种姓之间存在显着差异。使用触角和其他头部部位进行的实时 qPCR (RT-qPCR) 分析证实，这些基因在士兵触角中高度表达。最后，独立的 RT-qPCR 分析表明，这些基因的表达模式在来自不同社会背景的士兵中发生了改变。目前的结果表明，一些非受体的基因表达水平受到白蚁群体成员之间种姓和行为相互作用的影响。

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Gene expression profiles of chemosensory genes of termite soldier and worker antennae

Termites have an elaborate social system that involves cooperation and division of labour among colony members. Although this social system is regulated by chemical signals produced in the colony, it remains unclear how these signals are perceived by other members. Signal transduction is well known to be triggered by the reception of odorant molecules by some binding proteins in the antennae, after which, a signal is transmitted to chemosensory receptors. However, there is insufficient information on the role of chemosensory genes involved in signal transduction in termites. Here, we identified the genes involved in chemosensory reception in the termite Reticulitermes speratus and performed a genome-wide comparative transcriptome analysis of worker and soldier antennae. First, we identified 31 odorant-binding proteins (OBPs), and three chemosensory protein A (CheA) from the genome data. Thereafter, we performed RNA sequencing to compare the expression levels of OBPs, CheAs, and previously identified chemosensory receptor genes between worker and soldier antennae. There were no receptor genes with significant differences in expression between castes. However, the expression levels of three non-receptor odorant-detection/binding proteins (OBP, CheA, and Sensory neuron membrane protein) were significantly different between castes. Real-time qPCR (RT-qPCR) analysis using antennae and other head parts confirmed that these genes were highly expressed in soldier antennae. Finally, independent RT-qPCR analysis showed that the expression patterns of these genes were altered in soldiers from different social contexts. Present results suggest that gene expression levels of some non-receptors are affected by both castes and behavioural interactions among colony members in termites.

更新日期：2023-04-05

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