The development of highly efficient and stable electrocatalysts toward the acidic oxygen evolution reaction (OER) is essential for the practical application of proton-exchange membrane water electrolyzers. Although Ru oxides possess remarkable initial activity toward the acidic OER due to the kinetically favorable lattice oxygen oxidation mechanism pathway, the soluble high-valence oxygen-vacancy intermediate (∗Vo-RuO₄²⁻) may accelerate the dissolution of Ru species, leading to dramatically decreased activity and unsatisfied long-term stability. Here, we developed a robust metal-organic framework anchored strategy by stabilizing atomically isolated Ru oxide on UiO-67-bpydc with strong coordinating pyridine ligands. Theory calculations and experimental results including in situ Raman, X-ray absorption spectroscopy, and ¹⁸O-labeled differential electrochemical mass spectrometry reveal that the Ru–N bonds between Ru oxide and UiO-67-bpydc could not only accelerate the participation of lattice oxygen during the OER process but also stabilize the soluble ∗Vo-RuO₄²⁻ intermediate, which contribute to the enhanced OER performance and long-term stability of up to 115 h.