DNA 程序调节双金属纳米酶的生长和类酶活性及其生物传感应用,ACS Applied Materials & Interfaces

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DNA 程序调节双金属纳米酶的生长和类酶活性及其生物传感应用
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2023-04-05 , DOI: 10.1021/acsami.2c21854
Mengchao Cai ₁ , Yunqing Zhang ₁ , Zhongxu Cao ₁ , Wensong Lin ₁ , Na Lu ₁

Affiliation

纳米酶结合了纳米材料和天然酶的优点，作为人工酶模拟物的新代表受到了广泛关注。然而，合理设计纳米结构的形态和表面特性以产生所需的类酶活性仍然是一个巨大的挑战。在这里，我们报告了一种 DNA 编程种子生长策略，用于介导铂纳米粒子 (PtNPs) 在金双锥体 (AuBPs) 上的生长，用于合成双金属纳米酶。我们发现双金属纳米酶的制备具有序列依赖性，并且 polyT 序列的编码允许成功形成具有极大增强的过氧化物酶样活性的双金属纳米杂化物。我们进一步观察到 T15 介导的 Au/Pt 纳米结构 (Au/T15/Pt) 的形态和光学性质随反应时间而变化，并且可以通过控制实验条件来调整纳米酶活性。作为一个概念应用，Au/T15/Pt 纳米酶被用于建立一种简单、灵敏和选择性的比色法，用于测定抗坏血酸 (AA)、碱性磷酸酶 (ALP) 和抑制剂钒酸钠 (Na₃ VO ₄ ), 展示了出色的分析性能。这项工作为合理设计用于生物传感应用的双金属纳米酶提供了一条新途径。

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DNA-Programmed Tuning of the Growth and Enzyme-Like Activity of a Bimetallic Nanozyme and Its Biosensing Applications

Nanozymes, which combine the merits of both nanomaterials and natural enzymes, have aroused tremendous attention as new representatives of artificial enzyme mimics. However, it still remains to be a great challenge to rationally engineer the morphologies and surface properties of nanostructures that lead to the desired enzyme-like activities. Here, we report a DNA-programming seed-growth strategy to mediate the growth of platinum nanoparticles (PtNPs) on gold bipyramids (AuBPs) for the synthesis of a bimetallic nanozyme. We find that the preparation of a bimetallic nanozyme is in a sequence-dependent manner, and the encoding of a polyT sequence allows the successful formation of bimetallic nanohybrids with greatly enhanced peroxidase-like activity. We further observe that the morphologies and optical properties of T15-mediated Au/Pt nanostructures (Au/T15/Pt) change over the reaction time, and the nanozymatic activity can be tuned by controlling the experimental conditions. As a concept application, Au/T15/Pt nanozymes are used to establish a simple, sensitive, and selective colorimetric assay for the determination of ascorbic acid (AA), alkaline phosphatase (ALP), and the inhibitor sodium vanadate (Na₃VO₄), demonstrating excellent analytical performance. This work provides a new avenue for the rational design of bimetallic nanozymes for biosensing applications.

更新日期：2023-04-05

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