当前位置: X-MOL 学术Reprod. Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Molecular characterization, putative structure and function, and expression profile of OAS1 gene in the endometrium of goats (Capra hircus)
Reproductive Biology ( IF 2.5 ) Pub Date : 2023-04-04 , DOI: 10.1016/j.repbio.2023.100760
Asit Jain 1 , Tripti Jain 1 , Girish Kumar Mishra 1 , Khushboo Chandrakar 1 , Kishore Mukherjee 1 , Sita Prasad Tiwari 1
Affiliation  

An interferon-inducible gene, 2’-5’-oligoadenylate synthetase-1 (OAS1), plays an essential role in uterine receptivity and conceptus development by controlling cell growth and differentiation in addition to anti-viral activities. As OAS1 gene has not yet been studied in caprine (cp), so present study was designed with the aim to amplify, sequence, characterize and in-silico analyze the coding sequence of the cpOAS1. Further, expression profile of cpOAS1 was performed by quantitative real-time PCR and western blot in the endometrium of pregnant and cyclic does. An 890 bp fragment of the cpOAS1 was amplified and sequenced. Nucleotide and deduced amino acid sequences revealed 99.6–72.3% identities with that of ruminants and non-ruminants. A constructed phylogenetic tree revealed that Ovis aries and Capra hircus differ from large ungulates. Various post-translational modifications (PTMs), 21 phosphorylation, two sumoylation, eight cysteines and 14 immunogenic sites were found in the cpOAS1. The domain, OAS1_C, is found in the cpOAS1 which carries anti-viral enzymatic activity, cell growth, and differentiation. Among the interacted proteins with cpOAS1, Mx1 and ISG17 well-known proteins are found that have anti-viral activity and play an important role during early pregnancy in ruminants. CpOAS1 protein (42/46 kDa and/or 69/71 kDa) was detected in the endometrium of pregnant and cyclic does. Both cpOAS1 mRNA and protein were expressed maximally (P<0.05) in the endometrium during pregnancy as compared to cyclic does. In conclusion, the cpOAS1 sequence is almost similar in structure and probably in function also to other species along with its higher expression during early pregnancy.



中文翻译:

山羊 (Capra hircus) 子宫内膜中 OAS1 基因的分子特征、推定结构和功能以及表达谱

干扰素诱导基因 2'-5'-oligoadenylate synthetase-1 ( OAS1 ) 通过控制细胞生长和分化以及抗病毒活性在子宫容受性和孕体发育中发挥重要作用。由于尚未在山羊 (cp) 中研究OAS1基因,因此本研究旨在对cpOAS1的编码序列进行扩增、测序、表征和计算机分析此外, cpOAS1的表达谱是通过定量实时 PCR 和蛋白质印迹在怀孕和周期性子宫内膜中进行的。cpOAS1的 890 bp 片段被扩增和测序。核苷酸和推导的氨基酸序列揭示了与反刍动物和非反刍动物 99.6-72.3% 的同一性。构建的系统发育树表明,绵羊山羊不同于大型有蹄类动物在 cpOAS1 中发现了各种翻译后修饰 (PTM)、21 个磷酸化、2 个苏木化、8 个半胱氨酸和 14 个免疫原性位点。OAS1_C 域位于具有抗病毒酶活性的 cpOAS1 中、细胞生长和分化。在与 cpOAS1、Mx1 和 ISG17 相互作用的蛋白质中,发现具有抗病毒活性并在反刍动物早期妊娠期间发挥重要作用的知名蛋白质。在怀孕和周期性子宫内膜中检测到 CpOAS1 蛋白(42/46 kDa 和/或 69/71 kDa)。与周期性子宫内膜相比,cpOAS1 mRNA 和蛋白质在妊娠期间在子宫内膜中表达最多 (P<0.05)。总之, cpOAS1序列在结构上几乎相似,并且可能在功能上也与其他物种相似,而且它在早孕期间的表达更高。

更新日期:2023-04-05
down
wechat
bug