Cymbopogon citratus (DC.) Stapf (lemongrass or citronella) is a herb of therapeutic potential highly sought by various industries due to the presence of essential oil. In vitro propagation of commercial crops is highly desirable; it is an alternative approach for the production of quality planting materials. But the plantlets encounter certain morpho-structural and physiological disorders under in vitro conditions resulting in suppressed growth and reduced survival. The present study aimed to analyze the effect of silicon nanoparticles (SiNPs) on the in vitro morphometric and structural developments, rooting, and acclimatization efficiency in C. citratus. Murashige and Skoog (MS) medium supplemented with 4.0 mg/L of 6-benzylaminopurine (BAP) was effective for bud breaking. The shoot proliferation was further accelerated with the addition of 0.25 mg/L NAA (α-naphthalene acetic acid) in subsequent subcultures (after 3rd subculture, 21.0 shoots with 4.9 cm length per explants per culture vessel). Such shoots were characterized by thin leaf blades, reduced leaf area, and shoot tip necrosis, which negatively affected the life of cultures, ex vitro rooting, and acclimatization efficiency. Microscopic evaluation of those leaves showed underdeveloped stomata, epidermis, and reduced photosynthetic and vascular tissues. The incorporation of optimal concentration of SiNPs (40 mg/L) with the optimized growth regulators (4.0 mg/L BAP + 0.25 mg/L NAA) significantly increased shoot proliferation (38.0), shoot length (6.4 cm), robust leaf, and biomass (5.2 g fresh weight; 1.9 g dry weight). Shoot tip necrosis was reversed by the addition of SiNPs, and the developed shoots were morphologically thick with well-developed tissue systems. These C. citratus shoots performed better during ex vitro rooting (100% rooting, 8.0 roots/shoot) when treated with indole-3-butyric acid (IBA) at the concentration of 200 mg/L and acclimatization.

Cymbopogon citratus (DC.) Stapf（柠檬草或香茅）是一种具有治疗潜力的草本植物，由于含有精油，因此受到各行各业的高度追捧。经济作物的体外繁殖是非常可取的；这是生产优质种植材料的另一种方法。但是小植物在体外条件下会遇到某些形态结构和生理障碍，导致生长受抑制和存活率降低。本研究旨在分析硅纳米粒子 (SiNPs) 对柑桔离体形态和结构发育、生根和驯化效率. 补充有 4.0 mg/L 6-苄基氨基嘌呤 (BAP) 的 Murashige 和 Skoog (MS) 培养基可有效破芽。在随后的传代培养物中添加 0.25 mg/L NAA（α-萘乙酸）可进一步加速枝条增殖（第 3 次传代培养后，每个培养容器每个外植体有 21.0 个长 4.9 cm 的枝条）。此类芽的特征是叶片薄、叶面积减少和茎尖坏死，这对离体培养物的寿命产生负面影响生根和驯化效率。对这些叶子的显微镜评估显示气孔、表皮不发达，光合作用和维管组织减少。将最佳浓度的 SiNPs (40 mg/L) 与优化的生长调节剂 (4.0 mg/L BAP + 0.25 mg/L NAA) 结合显着增加了枝条增殖 (38.0)、枝条长度 (6.4 cm)、健壮的叶片和生物量（5.2 克鲜重；1.9 克干重）。通过添加 SiNPs 可以逆转芽尖坏死，并且发育的芽在形态上很厚，具有发育良好的组织系统。当用浓度为 200 mg/L 的吲哚-3-丁酸 (IBA) 处理和适应环境时，这些 C. citratus芽在体外生根（100% 生根，8.0 根/芽）期间表现更好。