基于便携式智能手机的微流控电化学免疫传感器，用于超灵敏检测人血清中的甲胎蛋白,Electroanalysis

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基于便携式智能手机的微流控电化学免疫传感器，用于超灵敏检测人血清中的甲胎蛋白
Electroanalysis ( IF 2.7 ) Pub Date : 2023-04-03 , DOI: 10.1002/elan.202300056
Qing Zhang ₁ , Yanyun Shen ₂ , Wenhui Lu ₂ , Tengfei Fu ₂ , keying zhang ₂ , Xin Zhuo ₂ , Hongyan Wang ₂ , Chao Liu ₂ , Xusheng Xie ₂ , Shangshang Ma ₂

Affiliation

快速准确地追踪生物标志物对于癌症的早期检测和诊断至关重要。因此，需要开发一种有效且方便的策略来有效监测癌症生物标志物。在此，我们构建了一种基于掺杂金纳米粒子（Au NP）的三维还原氧化石墨烯（3D rGO）的便携式微流电化学免疫传感器，用于超灵敏测定甲胎蛋白（AFP）。所设计的微流控芯片具有进样量小、结构可拆卸、集成度高等优点，采用3D打印技术制作而成，仅需9μL试剂即可实现高灵敏度检测。此外，3D Au NPs-rGO复合材料具有高比表面积和电子传输能力，可以有效增加电活性位点并增强电化学信号。受益于这些特性，3D Au NPs-rGO微流控电化学免疫芯片表现出0.1 pg/mL–200 ng/mL的宽检测范围，最佳检测限为0.045 pg/mL，灵敏度高达175.008 μA (ng/mL) ）^-1 厘米^-2。同时，所提出的免疫传感器在人血清样本中表现出可靠的 AFP 检测，这表明这种基于便携式智能手机的微流控电化学免疫传感器在临床检测方面具有巨大的前景，在个性化医疗保健方面具有巨大的潜力。

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Portable smartphone-based microfluidic electrochemical immunosensor for ultrasensitive detection of alpha-fetoprotein in human serum

Rapid and accurate tracing of biomarkers is essential for early detecting and diagnosing of cancer. Therefore, a valid and convenient strategy needs to be developed for efficient monitoring of cancer biomarkers. Herein, we constructed a portable microfluidic electrochemical immunosensor based on three-dimensional reduced graphene oxide (3D rGO) doped with gold nanoparticles (Au NPs) for ultrasensitive determination of alpha-fetoprotein (AFP). The designed microfluidic chip, with the advantages of small injection volume, detachable structure and high integration, was fabricated by 3D printing, which only needed 9 μL of reagent to realize the high sensitivity detection. In addition, the 3D Au NPs-rGO composites with high specific surface area and electrons transfer capacity can effectively increase electroactive sites and enhance electrochemical signals. Benefiting from these features, the 3D Au NPs-rGO microfluidic electrochemical immunochip showed a wide detection range between 0.1 pg/mL–200 ng/mL and a best detection limit of 0.045 pg/mL with the high sensitivity of 175.008 μA (ng/mL)⁻¹ cm⁻². Meanwhile, the proposed immunosensor exhibited reliable AFP detection in human serum samples, which demonstrated that this portable smartphone-based microfluidic electrochemical immunosensor hold great promises in clinical detection and huge potential in personalized healthcare.

更新日期：2023-04-03

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